{"title":"Multiomics reveals changes in lipid metabolism in the livers of landes geese before and after overfeeding.","authors":"Weiqing Ma, Liu Yang, Yadi Jing, Pengwei Ren, Xiang Liu, Meixia Zhang, Xiaomin Qi, Mingxia Zhu, Qiaomei Zhang","doi":"10.5713/ab.25.0405","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>: The aim of this experiment was to integrated production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding.</p><p><strong>Methods: </strong>: A total of 120 seven-week-old male Landes geese were floor reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150-180 g/meal) initially, gradually increasing to five meals (300-500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks.</p><p><strong>Results: </strong>: The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia-Shigella enrichment and norank_o_Clostridia_UCG-014 depletion.</p><p><strong>Conclusion: </strong>: Our work demonstrated that the identification of the \"gut microbiota‒bile acid‒liver axis\" could as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.25.0405","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: : The aim of this experiment was to integrated production indices with omics sequencing to elucidate the systemic perturbations between hepatic metabolism and the gut microbiota during overfeeding.
Methods: : A total of 120 seven-week-old male Landes geese were floor reared in a pen environment. Overfeeding commenced at week 8 using a corn-based diet containing 5% soybean oil. The feeding regimen consisted of three daily meals (150-180 g/meal) initially, gradually increasing to five meals (300-500 g/meal) after two weeks, and was maintained for a total overfeeding period of four weeks.
Results: : The results demonstrated that overfeeding significantly increased liver weight and serum lipid levels, accompanied by intracellular lipid droplet accumulation. Concurrently, the downregulation of taurine-conjugated bile acids and the upregulation of free bile acids disrupted cholesterol homeostasis. Crucially, overfeeding triggered gut microbial dysbiosis characterized by Escherichia-Shigella enrichment and norank_o_Clostridia_UCG-014 depletion.
Conclusion: : Our work demonstrated that the identification of the "gut microbiota‒bile acid‒liver axis" could as a pivotal signaling pathway driving overfeeding-induced foie gras formation while providing a theoretical foundation for overfeeding strategies to mitigate metabolic pathologies in waterfowl production.