Dynamic transcriptomic landscape of myogenesis in Muscovy ducks (Cairina moschata): Integrative analysis of hub genes post-hatching.

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Bioscience Pub Date : 2025-08-12 DOI:10.5713/ab.25.0159

Xiaofeng Li, Bing Yang

{"title":"Dynamic transcriptomic landscape of myogenesis in Muscovy ducks (Cairina moschata): Integrative analysis of hub genes post-hatching.","authors":"Xiaofeng Li, Bing Yang","doi":"10.5713/ab.25.0159","DOIUrl":null,"url":null,"abstract":"Objective‌: ‌: Post-hatching myogenesis is a critical determinant of meat yield and quality, with potential regulatory roles of specific genes remaining underexplored‌ in Muscovy ducks (Cairina moschata). This study aimed to identify hub genes governing post-hatching myogenesis through transcriptomic profiling‌.Methods‌: ‌: Three White-feathered male Muscovy ducks at 1-day-old (1D) and 80-day-old (80D) were selected, respectively. Following humane euthanasia, the left leg muscles were collected for subsequent RNA extraction, library construction, genome alignment, and transcriptome sequencing. DESeq2 (v1.16.1) was utilized to screen differentially expressed genes (DEGs) based on the criteria of |log2FoldChange| > 1 and P< 0.05 ‌‌. Functional enrichment (Gene Ontology, KEGG, PANTHER) and protein-protein interaction network analyses were conducted via DAVID, KOBAS, PANTHER, and STRING databases, with CytoHubba identifying myogenesis-associated hub genes‌.Results: ‌: A total of 1,683 DEGs were identified between 80D and 1D muscles, including 865 upregulated and 818 downregulated genes‌. Totally ten hub genes, such as CD3E, ITK, COL4A6, HADHB, MCCC2, and IVD, were prioritized as key regulators of myogenesis‌. Functional analysis revealed their enrichment in L-leucine catabolic processes and fatty acid β-oxidation‌. Pathway mapping further associated these genes with folate-mediated one-carbon metabolism, glycine/serine/threonine metabolism, and branched-chain amino acid degradation‌.Conclusion: ‌: This study delineates a molecular framework for post-hatching myogenesis in Muscovy ducks, highlighting ten hub genes that orchestrate myogenesis through metabolic reprogramming‌. These findings advance genetic strategies for improving poultry meat production and quality‌.","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.25.0159","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective‌: ‌: Post-hatching myogenesis is a critical determinant of meat yield and quality, with potential regulatory roles of specific genes remaining underexplored‌ in Muscovy ducks (Cairina moschata). This study aimed to identify hub genes governing post-hatching myogenesis through transcriptomic profiling‌.

Methods‌: ‌: Three White-feathered male Muscovy ducks at 1-day-old (1D) and 80-day-old (80D) were selected, respectively. Following humane euthanasia, the left leg muscles were collected for subsequent RNA extraction, library construction, genome alignment, and transcriptome sequencing. DESeq2 (v1.16.1) was utilized to screen differentially expressed genes (DEGs) based on the criteria of |log2FoldChange| > 1 and P< 0.05 ‌‌. Functional enrichment (Gene Ontology, KEGG, PANTHER) and protein-protein interaction network analyses were conducted via DAVID, KOBAS, PANTHER, and STRING databases, with CytoHubba identifying myogenesis-associated hub genes‌.

Results: ‌: A total of 1,683 DEGs were identified between 80D and 1D muscles, including 865 upregulated and 818 downregulated genes‌. Totally ten hub genes, such as CD3E, ITK, COL4A6, HADHB, MCCC2, and IVD, were prioritized as key regulators of myogenesis‌. Functional analysis revealed their enrichment in L-leucine catabolic processes and fatty acid β-oxidation‌. Pathway mapping further associated these genes with folate-mediated one-carbon metabolism, glycine/serine/threonine metabolism, and branched-chain amino acid degradation‌.

Conclusion: ‌: This study delineates a molecular framework for post-hatching myogenesis in Muscovy ducks, highlighting ten hub genes that orchestrate myogenesis through metabolic reprogramming‌. These findings advance genetic strategies for improving poultry meat production and quality‌.

查看原文本刊更多论文

番鸭（Cairina moschata）肌肉发生的动态转录组学景观：孵化后枢纽基因的综合分析。

目的：在麝香鸭（Cairina moschata）中，孵化后的肌肉发生是肉产量和品质的关键决定因素，特定基因的潜在调节作用尚未得到充分研究。本研究旨在通过转录组学分析确定控制孵化后肌肉发生的中心基因。方法：▪▪▪选取1日龄（1D）和80日龄（80D）的雄性白羽麝鸭3只。人道安乐死后，收集左腿肌肉进行RNA提取、文库构建、基因组比对和转录组测序。采用DESeq2 （v1.16.1）以|log2FoldChange| > 1和P< 0.05为标准筛选差异表达基因（DEGs）。功能富集（Gene Ontology， KEGG， PANTHER）和蛋白相互作用网络分析通过DAVID， KOBAS， PANTHER和STRING数据库进行，使用CytoHubba识别肌发生相关枢纽基因。结果：在80D和1D肌肉中共鉴定出1683个deg，包括865个上调基因和818个下调基因。共10个中心基因，如CD3E、ITK、COL4A6、HADHB、MCCC2和IVD，被优选为肌发生的关键调控因子。功能分析显示它们在l -亮氨酸分解代谢过程和脂肪酸β-氧化中富集。通路定位进一步将这些基因与叶酸介导的单碳代谢、甘氨酸/丝氨酸/苏氨酸代谢和支链氨基酸降解联系起来。结论：：本研究描绘了番鸭孵化后肌肉发生的分子框架，突出了通过代谢重编程协调肌肉发生的10个中心基因。这些发现促进了提高禽肉产量和质量的遗传策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊