Self-assembled aggregation-induced emission supramolecular probe for sensitive temozolomide detection and intracellular imaging

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-14 DOI:10.1007/s00216-025-06045-1

Chenrui Jiang, Feifei Chen, Yue Chen, Yu Sun, Hua He, Pierre Dramou, Tao Xu, Hongbin Xu

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引用次数: 0

Abstract

Temozolomide (TMZ) is a first-line chemotherapy drug for treating malignant glioblastoma but faces challenges due to its short plasma half-life and limited brain distribution. There is a need for a more efficient, cost-effective, and sensitive method for monitoring TMZ in clinical settings. This work presents a novel fluorescent probe based on the host–guest interaction of cucurbit[7]uril (CB[7]) and aggregation-induced emission molecule 1,1,2,2-tetra(biphenyl-4-yl)ethene (TTPE) for trace-level detection of TMZ in serum. The CB[7]@TTPE nanoprobe demonstrated high sensitivity, with a detection range of 1–20 μg/mL and a limit of detection of 0.25 μg/mL. Comprehensive characterization by ITC, FT-IR, NMR, UV–vis, and fluorescence spectroscopy confirmed the sensing behavior of the probe. The system exhibited excellent selectivity for TMZ and was successfully applied to serum samples with good recovery rates. In addition, the probe enabled fluorescence imaging of TMZ in human glioma cells (U87 and T98G), making it suitable for monitoring of drug distribution in cellular environments. The self-assembled CB[7]@TTPE probe provides a highly sensitive and selective method for TMZ detection in biological fluids. Its strong fluorescence and water solubility offer promising potential for both clinical diagnostics and drug resistance monitoring in cancer therapy.

Graphical Abstract

查看原文本刊更多论文

用于替莫唑胺敏感检测和细胞内成像的自组装聚集诱导发射超分子探针。

替莫唑胺（Temozolomide， TMZ）是治疗恶性胶质母细胞瘤的一线化疗药物，但由于其血浆半衰期短和脑分布有限而面临挑战。需要一种更有效、更经济、更灵敏的方法来监测临床环境中的TMZ。本研究提出了一种基于cucbit [7] （CB[7]）和聚集诱导发射分子1,1,2,2-四（联苯-4-基）乙烯（TTPE）主客体相互作用的新型荧光探针，用于血清中微量TMZ的检测。c[7]@TTPE纳米探针灵敏度高，检测范围为1 ~ 20 μg/mL，检出限为0.25 μg/mL。ITC, FT-IR， NMR， UV-vis和荧光光谱的综合表征证实了探针的传感行为。该系统对TMZ具有良好的选择性，并成功地应用于血清样品中，具有良好的回收率。此外，该探针能够对人胶质瘤细胞（U87和T98G）中的TMZ进行荧光成像，使其适用于监测细胞环境中的药物分布。自组装的CB b[7]@TTPE探针为生物流体中的TMZ检测提供了一种高灵敏度和高选择性的方法。它的强荧光性和水溶性为癌症治疗的临床诊断和耐药监测提供了良好的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.