Efficacy of Streptomyces achromogenes MAA01-influenced hydroxyapatite/graphene oxide nanocomposites for bone ossification.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-09-01 Epub Date: 2025-08-11 DOI:10.1007/s13205-025-04444-w

Kavitha Kandiah, Windu Negara, Priyadharsan Arumugam, Ramesh T Subramaniam, Murni Handayani, Chinnaperumal Kamaraj

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引用次数: 0

Abstract

This study investigates hydroxyapatite (HAp) nanocomposites infused with Streptomyces achromogenes MAA01-pretreated graphene oxide (GO) for bone tissue engineering. Hydrothermally synthesized HAp/GO (HH-G) nanocomposites showed rod with sheetlike structures with nano level crystallite size, and appropriate bioactivity and degradability. Raman spectroscopy confirmed the successful reduction of GO to reduced graphene oxide (rGO), enhancing antibacterial properties and cellular interactions. Among various formulations, the HH-G0.5 (1:0.5 ratio) nanocomposite demonstrated superior performance, promoting in vitro osteoblast proliferation, upregulating osteogenic gene markers (COL1, OCN, and ALP) in 2 folds, and exhibiting an appropriate Ca/P ratio (1.67) for optimal bone mineralization, as confirmed by in vivo analysis. The Streptomyces MAA01 pre-treatment notably improved antioxidant capacity and maintained a non-toxic profile. Histopathological evaluations showed non-significant toxicity or organ accumulation in liver and kidney tissues. In vivo implantation studies in Wistar rats further confirmed that HH-G0.5 significantly enhanced bone regeneration compared to pure GO or HAp. These results suggest that HH-G0.5 is a promising and safe and highly effective candidate for future clinical applications in bone tissue regeneration and repair.

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变色链霉菌maa01对羟基磷灰石/氧化石墨烯纳米复合材料骨成骨效果的影响

本研究研究了羟基磷灰石（HAp）纳米复合材料注入染色链霉菌maa01预处理氧化石墨烯（GO）用于骨组织工程。水热合成的羟基磷酰胺/氧化石墨烯（HH-G）纳米复合材料具有纳米级晶体大小的片状棒状结构，具有良好的生物活性和可降解性。拉曼光谱证实了氧化石墨烯成功还原为还原氧化石墨烯（rGO），增强了抗菌性能和细胞相互作用。在各种配方中，HH-G0.5（1:0.5比例）纳米复合材料表现出优异的性能，促进体外成骨细胞增殖，上调2倍的成骨基因标记（COL1、OCN和ALP），体内分析证实，钙磷比（1.67）适宜于最佳骨矿化。MAA01预处理显著提高了Streptomyces MAA01的抗氧化能力，并保持了其无毒特性。组织病理学评估显示肝脏和肾脏组织无明显毒性或器官积聚。Wistar大鼠体内植入研究进一步证实，与纯氧化石墨烯或HAp相比，HH-G0.5显著促进骨再生。这些结果表明HH-G0.5是一种有前景的、安全高效的骨组织再生修复候选材料。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.