Determination of Multi-Steroid Profiles of Cats With Hyperaldosteronism or Other Diseases: A Retrospective Study

IF 2.2 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2025-08-14 DOI:10.1111/jvim.70209

Alice H. Watson, Lorna C. Gilligan, Angela E. Taylor, Wiebke Arlt, Harriet M. Syme

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引用次数: 0

Abstract

Background

Multi-steroid profiles are proven to help with the diagnostic approach to adrenal disorders in humans.

Objectives

Compare liquid chromatography tandem mass spectrometry (LC–MS/MS) and radioimmunoassay (RIA) measurement of aldosterone in feline blood samples. Evaluate multi-steroid profiles in cats with hyperaldosteronism.

Animals

Client-owned cats with low/normal aldosterone by RIA (low aldo, n = 15), high aldosterone by RIA (high aldo, n = 6) and overt primary hyperaldosteronism (PHA, n = 6).

Methods

Exploratory retrospective case–control study. Feline blood multi-steroid profiles were analyzed with LC–MS/MS and compared with results from a commercially available RIA in widespread clinical use.

Results

Aldosterone measurement by LC–MS/MS and RIA was strongly correlated (r = 0.93, p < 0.001) but showed a bias of 56 (95% CI: 104–7) pmol/L, with RIA giving higher values. Creatinine concentration was not significantly associated with the discrepancy between methods (p = 0.95). Cats with PHA had significantly lower serum concentrations of cortisol and glucocorticoid precursors (17-hydroxyprogesterone, 11-deoxycortisol), testosterone, and significantly higher dihydrotestosterone and 5α-dihydroprogesterone concentrations than the low aldo group. Pregnenolone concentrations were significantly lower in the PHA group than in the high aldo group.

Conclusion and Clinical Importance

Quantification of aldosterone by either LC–MS/MS or RIA is adequate for PHA diagnosis in the clinical setting, with appropriate method-specific reference intervals. Cats with PHA had different concentrations of some steroids compared with the control groups, but overt steroid excess (other than aldosterone) was not observed.

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测定患有高醛固酮增多症或其他疾病的猫的多种类固醇谱：一项回顾性研究

背景：多种类固醇谱被证明有助于人类肾上腺疾病的诊断方法。目的比较液相色谱串联质谱法（LC-MS /MS）和放射免疫分析法（RIA）测定猫血中醛固酮的方法。评估高醛固酮增多症猫的多种类固醇概况。客户所拥有的猫，经RIA诊断为醛固酮低/正常（低aldo， n = 15）、经RIA诊断为醛固酮高（高aldo， n = 6）和明显的原发性醛固酮增多症（PHA, n = 6）。方法探索性回顾性病例对照研究。采用LC-MS /MS对猫血液中的多种类固醇谱进行分析，并与临床广泛使用的市售RIA结果进行比较。结果hplc - MS/MS法测定醛固酮与RIA法测定的醛固酮浓度呈强相关（r = 0.93, p < 0.001），但偏差为56 (95% CI: 104-7) pmol/L， RIA法测定的醛固酮浓度较高。肌酐浓度与方法间差异无显著相关性（p = 0.95）。与低aldo组相比，患有PHA的猫血清皮质醇和糖皮质激素前体（17-羟基孕酮，11-脱氧皮质醇）、睾酮浓度显著降低，双氢睾酮和5α-双氢孕酮浓度显著升高。PHA组孕烯醇酮浓度明显低于高aldo组。结论和临床意义LC-MS /MS或RIA定量醛固酮在临床诊断PHA时是足够的，有适当的方法特异性参考区间。与对照组相比，患有PHA的猫有不同浓度的类固醇，但没有观察到明显的类固醇过量（醛固酮除外）。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.