Liquid Exfoliated Graphene/Ti3C2 MXene Composite Based Aptasensor for Ultrasensitive Electrochemical Detection of Chloramphenicol in Milk and Honey

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-07-10 DOI:10.1007/s12161-025-02856-1

Panpan Dong, Fang Li, Zijian Wu, Lu Xue, Mingming Luo, Yijun Tan, Haitao Li, Xu Yang

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引用次数: 0

Abstract

The presence of chloramphenicol (CAP) residues in animal-derived foods has the potential to accumulate in the human body during metabolism, which can result in adverse effects on human health. Herein, a novel ultrasensitive detection platform based on liquid exfoliated graphene (LEG)/Ti₃C₂ MXene nanocomposites modified indium tin oxide (ITO) electrode (LEG/Ti₃C₂ MXene/ITO), is proposed for the detection of CAP. The embedding of LEG into Ti₃C₂ MXene can suppress the aggregation of Ti₃C₂ MXene flakes, which has been proven to be an effective method for improving sensing performance due to its advantages of higher specific surface area and electron transfer efficiency. The CAP-specific aptamer is immobilized on LEG/Ti₃C₂ MXene/ITO through π-π adsorption as the bio-recognition element. Differential pulse voltammograms are utilized to analyze the changes in peak current of the modified electrode before and after the interaction between the immobilized aptamer and CAP, achieving quantitative measurement of CAP. The prepared aptamer/LEG/Ti₃C₂ MXene/ITO sensor has allowed the linear concentration range of CAP from 0.001 pM to10 μM, with a limit of detection (LOD) of 0.485 fM. The sensor performs well in stability, reproducibility, and selectivity. Additionally, it is demonstrated successfully to detect CAP in the spiked samples of milk and honey.

Graphical Abstract

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液体剥脱石墨烯/Ti3C2 MXene复合传感器用于牛奶和蜂蜜中氯霉素的超灵敏电化学检测

动物源性食品中存在的氯霉素（CAP）残留有可能在人体代谢过程中积累，从而对人体健康产生不利影响。本文提出了一种基于液体剥离石墨烯(LEG)/Ti3C2 MXene纳米复合材料修饰氧化铟锡（ITO）电极（LEG/Ti3C2 MXene/ITO）的新型超灵敏检测平台，用于CAP的检测。LEG嵌入Ti3C2 MXene中可以抑制Ti3C2 MXene薄片的聚集，由于其具有更高的比表面积和电子传递效率，已被证明是提高检测性能的有效方法。通过π-π吸附将cap特异性适配体固定在LEG/Ti3C2 MXene/ITO上作为生物识别元件。利用差分脉冲伏安分析修饰电极在固定适配体与CAP相互作用前后的峰值电流变化，实现了CAP的定量测量。制备的适配体/LEG/Ti3C2 MXene/ITO传感器允许CAP的线性浓度范围为0.001 pM ~ 10 μM，检出限（LOD）为0.485 fM。该传感器具有良好的稳定性、重复性和选择性。此外，该方法还成功地检测了牛奶和蜂蜜样品中的CAP。图形抽象

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.