Association of physical activity and hydration markers with cellular health in older adults: A doubly labeled water study

IF 4.3

Experimental gerontology Pub Date : 2025-08-11 DOI:10.1016/j.exger.2025.112859

Kan Oishi , Tsukasa Yoshida , Hyeon-Ki Kim , Keiichi Yokoyama , Yuya Watanabe , Yui Nakayama , Misaka Kimura , Kojiro Ishii , Rei Ono , Yosuke Yamada

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Abstract

Purpose

This study aimed to determine the associations between the phase angle (PhA), extracellular water-to-intracellular water ratio (ECW/ICW), physical activity levels (PALs) measured using the doubly labeled water (DLW) method, and the time spent engaging in physical activities of varying intensities in older adults living in Japan.

Methods

This study was part of the Kyoto–Kameoka prospective cohort study of older adults. The PhA and ECW/ICW ratio were measured using a multi-frequency bioelectrical impedance analysis device. PAL, percentage body fat, and fat-free mass index were calculated using the DLW method, while the average daily moderate-to-vigorous physical activity (MVPA), high-intensity light physical activity (HLPA), low-intensity light physical activity, and sedentary behavior durations were quantified using a triaxial accelerometer.

Results

Adjusted partial order correlation analysis showed significant positive correlations between the PhA and PAL (p = 0.001), as well as between the PhA and the MVPA (p = 0.027) and HLPA (p = 0.023) durations. The PAL was also significantly negatively correlated with the ECW/ICW ratio (p = 0.008). Adjusted multiple regression analysis confirmed that PAL (p = 0.004) and HLPA (p = 0.025) were significantly positively associated with PhA and negatively associated with ECW/ICW ratio (p = 0.004 and p = 0.036, respectively).

Conclusions

PAL, measured using the precise DLW method, is an explanatory factor for PhA and ECW/ICW ratio. HLPA below the MVPA threshold was linked to more favorable hydration markers of cellular health in older adults. As this was a cross-sectional study, causality cannot be inferred.

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老年人身体活动和水合标志物与细胞健康的关系：一项双标记水研究

目的本研究旨在确定日本老年人的相位角（PhA）、细胞外水/细胞内水比（ECW/ICW）、双标记水（DLW）法测量的身体活动水平（PALs）与参与不同强度体育活动的时间之间的关系。方法：本研究是老年人Kyoto-Kameoka前瞻性队列研究的一部分。采用多频生物电阻抗分析装置测定PhA和ECW/ICW比值。使用DLW法计算PAL、体脂百分比和无脂质量指数，同时使用三轴加速度计量化平均每日中高强度体力活动（MVPA）、高强度光体力活动（HLPA）、低强度光体力活动和久坐行为持续时间。结果经调整偏序相关分析，PhA与PAL、MVPA （p = 0.027）、HLPA （p = 0.023）时间呈正相关（p = 0.001）。PAL与ECW/ICW比值呈显著负相关（p = 0.008）。校正多元回归分析证实PAL （p = 0.004）和HLPA （p = 0.025）与PhA呈正相关，与ECW/ICW负相关（p = 0.004和p = 0.036）。结论采用精确的DLW法测量的spal是PhA和ECW/ICW比值的一个解释因素。在老年人中，低于MVPA阈值的HLPA与更有利的细胞健康水合标志物有关。由于这是一项横断面研究，因此无法推断因果关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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