Effect of Abelmoschus manihot (L.) Medik chloroform leaf extract on streptozotocin-induced diabetic rats

Pharmacological Research - Natural Products Pub Date : 2025-08-09 DOI:10.1016/j.prenap.2025.100335

Selvaraj Divya , Subramanian Arivoli , Samuel Tennyson

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Abstract

Ethnomedicine is a potentially fruitful area of study in treating diabetes since plants are reported to possess antidiabetic properties. In this study, Abelmoschus manihot leaf chloroform extract (AMLCE) was assessed for its antidiabetic effect on streptozotocin (STZ)-induced diabetic Wistar albino rats. The experimental design comprised of seven groups with six rats in each group, viz., Group I: Normal control, Group II: STZ (Diabetic control), Group III: STZ + AMLCE [100 mg/Kg BW], Group IV: STZ + AMLCE [200 mg/Kg BW], Group V: STZ + AMLCE [400 mg/Kg BW], Group VI: STZ + Glibenclamide [600 µg/Kg BW], and Group VII: Normal control + AMLCE [400 mg/Kg BW]. Body weight of Group I, II and V were 207.23 ± 8.99, 121.98 ± 7.61 and 162.82 ± 7.18 g, respectively; and their respective plasma glucose levels were 95.31 ± 4.53, 286.93 ± 4.82 and 126.88 ± 7.14 mg/dL. The insulin and total haemoglobin levels of Group I, II and V were 16.62 ± 1.10, 7.20 ± 2.60, 11.90 ± 1.72 µU/mL; and 12.60 ± 1.68, 6.51 ± 0.62, 10.17 ± 0.54 g/dL, respectively. When compared to the STZ-induced diabetic Wistar albino rats, AMLCE significantly increased the body weight and levels of insulin levels and total haemoglobin (P≤0.05) and decreased plasma glucose level (P≤0.05). AMLCE Group V was taken up for further studies since it showed a noteworthy impact in comparison to the other two groups of AMLCE. The histopathological study revealed STZ-induced diabetic Wistar albino rats treated with AMLCE with restoration of β-cells in islet of Langerhans in pancreas, regeneration of hepatocytes in liver, and restoration of glomerulus tubules in kidney. The expression of IR, PI3K, p-Akt, and GLUT-2 protein levels in STZ-induced diabetic Wistar albino rats increased upon treatment with AMLCE. The findings of this investigation suggest that AMLCE exhibited anti-diabetic effect. This study represents the first report on the anti-diabetic effects of AMLCE on STZ-induced diabetic Wistar albino rats.

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马氏白僵鼠的防治效果三氯甲烷叶提取物对链脲佐菌素诱导的糖尿病大鼠的作用

民族医学是治疗糖尿病的一个潜在的富有成果的研究领域，因为据报道植物具有抗糖尿病的特性。本研究以链脲佐菌素（STZ）诱导的糖尿病Wistar白化大鼠为实验对象，研究了马尼沙叶氯仿提取物（AMLCE）的降糖作用。实验设计组成的7组,每组六个老鼠,即,组我:正常的控制,组2:STZ(糖尿病控制),第三组:STZ + AMLCE( 100毫克/公斤体重),第四组:STZ + AMLCE( 200毫克/公斤体重),集团V: STZ + AMLCE( 400毫克/公斤体重),第六组:STZ + 格列本脲[600 µg / Kg BW),和组七:正常控制+ AMLCE( 400毫克/公斤体重)。I、II、V组体重分别为207.23 ± 8.99、121.98 ± 7.61和162.82 ± 7.18 g；血糖分别为95.31 ± 4.53、286.93 ± 4.82和126.88 ± 7.14 mg/dL。I、II、V组胰岛素和总血红蛋白水平分别为16.62 ± 1.10、7.20 ± 2.60、11.90 ± 1.72µU/mL；1.68, 6.51和12.60 ±  ± 0.62,10.17 ±0.54  g / dL,分别。与stz诱导的糖尿病Wistar白化大鼠相比，AMLCE显著提高体重、胰岛素水平和总血红蛋白水平（P≤0.05），显著降低血糖水平（P≤0.05）。由于与其他两组AMLCE相比，AMLCE V组表现出显著的影响，因此进一步进行了研究。组织病理学研究显示，stz诱导的糖尿病Wistar白化大鼠经AMLCE处理后，胰腺朗格汉斯岛β-细胞恢复，肝脏肝细胞再生，肾脏肾小球小管恢复。AMLCE治疗后，stz诱导的糖尿病Wistar白化大鼠IR、PI3K、p-Akt和GLUT-2蛋白表达水平升高。本研究结果提示AMLCE具有抗糖尿病作用。本研究首次报道了AMLCE对stz诱导的糖尿病Wistar白化大鼠的抗糖尿病作用。

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Pharmacological Research - Natural Products

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