The effect of heat stress on paper wasps from different climates

IF 2.9 2区 生物学 Q2 BIOLOGY
Astrid Bay Amstrup , Helmut Kovac , Helmut Käfer , Anton Stabentheiner , Jesper Givskov Sørensen
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Abstract

In a warming world, mechanisms for coping with stressfully high temperatures are of great importance to ectotherms such as insects. Among these mechanisms is the Heat Shock Response, which aids in recovery from heat stress and increases thermal resistance. The primitively eusocial paper wasps (Polistes spp.) are found across widely varying thermal environments, showcasing their adaptability with regard to temperature. We investigated the Heat Shock Response in paper wasp adults by measuring the expression of heat shock proteins (hsp70, hsp83, hsc70) under control conditions (22–24 °C) or following a heat exposure (42 °C or 44 °C). Additionally, we measured heat stress resistance as time to knockdown. The wasps were collected from three climatic regions (Alpine, temperate, Mediterranean) and included three closely related species (P. biglumis, P. dominula, P. gallicus) to investigate if they used the Heat Shock Response differently. We found evidence of species-specific basal expression under control conditions, with the Mediterranean P. gallicus showing the highest expression. Both hsp70 and hsp83 were found to be highly responsive to heat stress. The results of the time to knockdown assay indicated a difference in both basal tolerance and in the response to heat stress between species and climates of origin. Overall, the study shows the multifunctionality of the Heat Shock Response, as the utilization differed between species depending on geographical origins. The results suggest that a quick adaptive utilization of the Heat Shock Response could be one of the physiological traits allowing paper wasp species to inhabit such widely different thermal environments.
热应力对不同气候条件下的纸蜂的影响
在一个变暖的世界里,应对高温压力的机制对昆虫等变温动物来说非常重要。这些机制之一是热休克反应,它有助于从热应激中恢复并增加热阻。原始的社会纸黄蜂(Polistes spp.)在广泛变化的热环境中被发现,显示出它们对温度的适应性。我们通过测量热休克蛋白(hsp70, hsp83, hsc70)在对照条件(22-24°C)或热暴露(42°C或44°C)下的表达来研究纸黄蜂成虫的热休克反应。此外,我们还测量了热应力抵抗时间。从三个气候区(高山、温带和地中海)收集黄蜂,包括三个密切相关的物种(P. biglumis, P. dominula, P. gallicus),以研究它们对热休克反应的不同反应。在对照条件下,我们发现了物种特异性基础表达的证据,其中地中海的P. gallicus表达最高。hsp70和hsp83对热应激均有较高的反应。敲除时间测定的结果表明,在物种和原产地气候之间,基础耐受性和对热胁迫的反应都存在差异。总的来说,该研究显示了热休克反应的多功能性,因为不同物种的利用因地理来源而异。研究结果表明,对热休克反应的快速适应性利用可能是纸黄蜂能够在如此广泛不同的热环境中生存的生理特征之一。
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来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
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