Expeditious and Automated Dispensing of ChemBeads for High-Throughput Experimentation in 384-Well Microtiter Plates

IF 3.5 3区化学 Q2 CHEMISTRY, APPLIED

Organic Process Research & Development Pub Date : 2025-07-23 DOI:10.1021/acs.oprd.5c00232

Vincent Porte*, Philipp Hirschle*, Kilian Frank, Luca Hepp, Philipp Kollmus, Eloisa Serrano, Christian Ziegler*, Roland Heinrich, Simon Seebacher, Christoph Weiss, James M. Fordham and Marco Santagostino*,

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引用次数: 0

Abstract

A unique automated dispensing robot to dose chemical-coated beads in microtiter plates (MTPs) at unprecedented speed is reported. With its novel dosing head design, ChemBeads can be reliably dosed volumetrically into 96- and 384-well MTPs. The platform features an intuitive graphical user interface, rendering the dosing protocol easy and flexible while ensuring traceability. This technology eliminates the need for multiple precatalyst/ligand stock solutions and overcomes the time constraint typically associated with automated gravimetric solid dosing. We also introduce a general workflow for performing metal-catalyzed reactions in 384-well glass MTPs under demanding conditions. The streamlined process was validated with a challenging C–N cross coupling reaction, conducted at elevated temperatures under heterogeneous conditions and using solvents rarely utilized in ultrahigh-throughput experimentation.

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快速和自动分配化学珠高通量实验在384孔微量滴定板

报道了一种独特的自动点药机器人，以前所未有的速度在微量滴度板（MTPs）中给化学包被珠进行剂量。凭借其新颖的定量头设计，ChemBeads可以可靠地对96井和384井的MTPs进行定量。该平台具有直观的图形用户界面，使给药协议简单灵活，同时确保可追溯性。该技术消除了对多种预催化剂/配体原液的需求，并克服了自动重量固体加药的时间限制。我们还介绍了在苛刻条件下在384孔玻璃MTPs中进行金属催化反应的一般工作流程。该流程通过具有挑战性的C-N交叉偶联反应进行了验证，该反应在非均相条件下在高温下进行，并且使用了在超高通量实验中很少使用的溶剂。

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来源期刊

Organic Process Research & Development 化学-应用化学

CiteScore

6.90

自引率

14.70%

发文量

251

审稿时长

2 months

期刊介绍： The journal Organic Process Research & Development serves as a communication tool between industrial chemists and chemists working in universities and research institutes. As such, it reports original work from the broad field of industrial process chemistry but also presents academic results that are relevant, or potentially relevant, to industrial applications. Process chemistry is the science that enables the safe, environmentally benign and ultimately economical manufacturing of organic compounds that are required in larger amounts to help address the needs of society. Consequently, the Journal encompasses every aspect of organic chemistry, including all aspects of catalysis, synthetic methodology development and synthetic strategy exploration, but also includes aspects from analytical and solid-state chemistry and chemical engineering, such as work-up tools，process safety, or flow-chemistry. The goal of development and optimization of chemical reactions and processes is their transfer to a larger scale; original work describing such studies and the actual implementation on scale is highly relevant to the journal. However, studies on new developments from either industry, research institutes or academia that have not yet been demonstrated on scale, but where an industrial utility can be expected and where the study has addressed important prerequisites for a scale-up and has given confidence into the reliability and practicality of the chemistry, also serve the mission of OPR&D as a communication tool between the different contributors to the field.