CD44 and Snail1 Expression Predicts Poor Prognosis of Oral Squamous Cell Carcinoma.

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-08-12 DOI:10.1111/jop.70032

Cintia Eliza Marques, Everton Freitas de Morais, Bruno Cesar da Costa, Fábio Haach Téo, Ana Lúcia Carrinho Ayroza Rangel, Ricardo D Coletta, Lívia Maris Ribeiro Paranaiba Dias

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引用次数: 0

Abstract

Background: Oral squamous cell carcinoma (OSCC) remains a challenging malignancy with poor 5-year survival rates due to diagnosis at an advanced stage and a high likelihood of recurrence and metastasis. These aggressive traits may be influenced by cancer stem cells (CSC) and epithelial-mesenchymal transition (EMT).

Methods: This study investigated the prognostic significance of the CSC marker CD44 and EMT-related proteins (Snail1, Snail2, E-cadherin, N-cadherin) in 132 OSCCs using immunohistochemistry. The comprehensive survival analysis included univariate and multivariate (stepwise method) Cox regression for disease-specific survival (DSS) and disease-free survival (DFS), Kaplan-Meier curves based on log-rank testing, and receiver operating characteristic (ROC) analysis to assess the predictive accuracy of the markers.

Results: High CD44 expression independently predicted worse DSS (HR = 2.74, 95% CI 1.44-5.23, p = 0.003) and DFS (HR = 2.22, 95% CI 1.16-4.23, p = 0.01), and Snail1 was significantly associated with poor DSS (HR = 2.62, 95% CI 1.37-5.03, p = 0.004). The combined expression of CD44 and Snail1 improved the discrimination of worse outcomes compared to markers individually. The presence of lymphovascular invasion (HR = 8.68, 95% CI 3.81-19.75, p < 0.0001) and a positive surgical margin (< 5 mm; HR = 4.45, 95% CI 1.99-9.96, p = 0.0003) were also independently associated with DSS.

Conclusions: The results of this study highlight the prognostic significance of CD44 and Snail1 in OSCC, emphasizing their potential interplay in tumor aggressiveness.

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CD44和Snail1表达预测口腔鳞状细胞癌预后不良。

背景：口腔鳞状细胞癌（OSCC）仍然是一种具有挑战性的恶性肿瘤，由于诊断为晚期，复发和转移的可能性高，5年生存率低。这些侵袭性特征可能受到癌症干细胞（CSC）和上皮-间质转化（EMT）的影响。方法：应用免疫组化方法研究132例OSCCs中CSC标志物CD44及emt相关蛋白（Snail1、Snail2、E-cadherin、N-cadherin）的预后意义。综合生存分析包括单因素和多因素（逐步法）疾病特异性生存（DSS）和无病生存（DFS）的Cox回归，基于log-rank检验的Kaplan-Meier曲线，以及评估标志物预测准确性的受试者工作特征（ROC）分析。结果：CD44高表达独立预测较差的DSS （HR = 2.74, 95% CI 1.44-5.23, p = 0.003）和DFS (HR = 2.22, 95% CI 1.16-4.23, p = 0.01)， Snail1与较差的DSS相关（HR = 2.62, 95% CI 1.37-5.03, p = 0.004）。与单独标记物相比，CD44和Snail1的联合表达提高了对不良结果的区分。淋巴血管浸润的存在（HR = 8.68, 95% CI 3.81-19.75, p）结论：本研究结果强调了CD44和Snail1在OSCC中的预后意义，强调了它们在肿瘤侵袭性中的潜在相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.