Surface plasmon resonance sensor for direct oxytetracycline detection with a newly selected aptamer

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-13 DOI:10.1007/s00216-025-06052-2

Meiting Kang, Liqing Wu, Qiang Zhao

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Abstract

The overuse of tetracycline antibiotics can lead to its residue in the aquatic ecological environment and subsequently cause risks to human health through the food chain. Therefore, effective determination of oxytetracycline (OTC) residues is urgently needed. In this study, a simple and direct aptamer-based surface plasmon resonance (SPR) sensor has been fabricated and applied for selective and sensitive detection of the antibiotic OTC by using the newly reported short aptamer for OTC. Biotinylated aptamers were immobilized on the surface of a SPR chip functionalized with streptavidin. When OTC bound to its aptamer, the refractive index of the SPR chip changed, and the SPR signal response increased. The SPR chip could be regenerated by simply flowing the running buffer without the need for an additional regeneration solution. We found that a high density of aptamer immobilized on the SPR chip allowed for an enhanced SPR response to OTC. This aptamer SPR sensor had a wide dynamic range of 1.56 nM to 3.2 µM for OTC detection with a detection limit of 3.6 nM. This SPR sensor can be reused and shows high stability. This method demonstrated good specificity towards OTC and enabled us to detect OTC in diluted lake water, showing applicability for OTC detection in practical applications.

Graphical Abstract

Abstract Image

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表面等离子体共振传感器直接检测土霉素与新选择的适配体。

四环素类抗生素的过度使用会导致其在水生生态环境中残留，进而通过食物链对人类健康造成危害。因此，迫切需要对土霉素（OTC）进行有效的残留检测。本研究利用新报道的OTC短适体，制备了一种简单、直接的基于表面等离子体共振（SPR）的传感器，用于抗生素OTC的选择性、敏感性检测。将生物素化的适配体固定在链霉亲和素功能化的SPR芯片表面。当OTC与其适配体结合时，SPR芯片的折射率发生变化，SPR信号响应增加。SPR芯片可以通过简单地流动运行缓冲来再生，而不需要额外的再生解决方案。我们发现高密度的适体固定在SPR芯片上，可以增强SPR对OTC的响应。该传感器具有1.56 nM ~ 3.2µM的动态范围，检测限为3.6 nM。该SPR传感器可重复使用，稳定性高。该方法对OTC具有良好的特异性，能够在稀释的湖水中检测OTC，在实际应用中具有一定的适用性。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.