Drainage Reorganization and Intraspecific Genetic Diversity of Riverine Fish in the Ligurian Alps and Northern Apennines

Abstract

Mountain building reorganizes drainage networks, influencing riverine biodiversity. Northern Italy offers a natural experiment in the impact of tectonic and geomorphic processes on aquatic species distribution. We combined geomorphic analysis with environmental DNA from rivers to assess the influence of tectonically driven drainage reorganization on genetic diversity, targeting an endemic fish species, Telestes muticellus (Risso et al., 1826, https://www.biodiversitylibrary.org/bibliography/58984). In the Northern Apennines, horizontal shortening and topographic advection in an orogenic wedge have been hypothesized as leading to river capture and drainage divide migration. In addition, slab rollback has produced a spatial transition from contraction to extension that is more pronounced from north to south, with normal faulting producing range-parallel drainage only in the southern regions. In contrast, the adjacent Ligurian Alps are a remnant of the Alpine orogen with little modern deformation. We found distinct zones of geomorphic characteristics from north to south, including divide asymmetry and frequency of range-parallel drainage. Analysis of DNA sequences shows cross-divide assemblage characteristics that correlate with the geomorphic zonation. In terms of directional measures of assemblage change, the Northern Apennines show higher values of overlap, gain, loss, turnover, and nestedness than those in the Ligurian Alps. Main drainage divide asymmetry correlates positively with genetic distance and gain, loss, and turnover of DNA sequences from Adriatic to Ligurian sites and negatively with overlap and nestedness. Since the species is confined to freshwater environments, tectonically driven drainage reorganization can explain its spatial genetic differentiation.