Reverse Micelles Dually Influence the Efficient Inhibition of the Heat-Induced Oxidation of Camellia Oil by Epigallocatechin Gallate and Epigallocatechin

Abstract

Reverse micelles ubiquitously assemble in the presence of water and amphiphilic substances in edible oils and are the primary locations for oxidation-related reactions. This study revealed the role of reverse micelles in controlling the efficiencies of epigallocatechin gallate (EGCG) and epigallocatechin (EGC) in suppressing the heating-induced deterioration of camellia oil. Using hydroperoxides, conjugated dienes, and carbonylic compounds, we determined that reverse micelles could dually regulate the efficiencies of EGCG and EGC in suppressing the degradation of camellia oil. In particular, reverse micelles in an aliquot containing 500 µmol/kg EGC positively controlled EGC activity; however, those containing 500, 10, and 10 µmol/kg of EGCG, EGC, and EGCG, respectively, showed negative effects to corresponding phenolic compounds. Thus, the influence of reverse micelles depends on the concentration and polarity of phenolic compounds. This study provides a new perspective for the development of antioxidant strategies for camellia oil.

Practical Application: This study highlights the critical role of reverse micelles in modulating the antioxidant efficiency of epigallocatechin gallate (EGCG) and epigallocatechin (EGC) in camellia oil. Understanding how reverse micelles influence these antioxidants’ activity depending on their concentration and polarity provides valuable insight for optimizing antioxidant formulations in edible oils. These results can guide the design of more effective antioxidant delivery systems or processing conditions to enhance the oxidative stability and shelf life of camellia oil and potentially other edible oils.