Automated Transcranial Photobiomodulation in Healthy Rats: Effects on Working Memory and Brain Markers

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Candela Zorzo, Lucía Rodríguez-Fernández, Emily Gabriela Castillo-Escalona, Juan A. Martínez, Laura Mañas, Natalia Arias, Jorge L. Arias
{"title":"Automated Transcranial Photobiomodulation in Healthy Rats: Effects on Working Memory and Brain Markers","authors":"Candela Zorzo,&nbsp;Lucía Rodríguez-Fernández,&nbsp;Emily Gabriela Castillo-Escalona,&nbsp;Juan A. Martínez,&nbsp;Laura Mañas,&nbsp;Natalia Arias,&nbsp;Jorge L. Arias","doi":"10.1002/adtp.202500002","DOIUrl":null,"url":null,"abstract":"<p>Photobiomodulation (PBM) uses red and near-infrared light to stimulate biological processes through cytochrome c oxidase (CCO) activation, enhancing ATP synthesis and neuroprotection. This study evaluates the PBM effects on spatial working memory (WM) and cellular mechanisms in healthy adult male rats, focusing on CCO activity, c-Fos, and synaptogenesis-related proteins. PBM (810 nm, 40 Hz, 20 J cm<sup>−2</sup>) is applied for five consecutive days (PBM-C) or five alternating days (PBM-A). PBM improves spatial WM in both groups compared to controls. CCO activity decreases in the prefrontal and retrosplenial cortex, as well as in the hippocampus, suggesting more efficient energy use during cognitive tasks. PBM increases c-Fos expression in the prefrontal and parietal cortex, reflecting heightened neuronal activity. Synapsin-I levels rise in the prefrontal cortex for both protocols, while PBM-C increases PSD-95 in the hippocampus. GFAP expression decreases in cortical regions with both protocols, while PBM-C increases it in the prefrontal cortex. These findings suggest that PBM PBM-C enhances prefrontal and hippocampal synapses, potentially underlying observed WM improvements. This study highlights the PBM potential in modulating CCO activity and synaptic plasticity, providing a basis for identifying effective schedules and targets for WM preservation and treatment.</p>","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 8","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202500002","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Therapeutics","FirstCategoryId":"3","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/adtp.202500002","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0

Abstract

Photobiomodulation (PBM) uses red and near-infrared light to stimulate biological processes through cytochrome c oxidase (CCO) activation, enhancing ATP synthesis and neuroprotection. This study evaluates the PBM effects on spatial working memory (WM) and cellular mechanisms in healthy adult male rats, focusing on CCO activity, c-Fos, and synaptogenesis-related proteins. PBM (810 nm, 40 Hz, 20 J cm−2) is applied for five consecutive days (PBM-C) or five alternating days (PBM-A). PBM improves spatial WM in both groups compared to controls. CCO activity decreases in the prefrontal and retrosplenial cortex, as well as in the hippocampus, suggesting more efficient energy use during cognitive tasks. PBM increases c-Fos expression in the prefrontal and parietal cortex, reflecting heightened neuronal activity. Synapsin-I levels rise in the prefrontal cortex for both protocols, while PBM-C increases PSD-95 in the hippocampus. GFAP expression decreases in cortical regions with both protocols, while PBM-C increases it in the prefrontal cortex. These findings suggest that PBM PBM-C enhances prefrontal and hippocampal synapses, potentially underlying observed WM improvements. This study highlights the PBM potential in modulating CCO activity and synaptic plasticity, providing a basis for identifying effective schedules and targets for WM preservation and treatment.

Abstract Image

Abstract Image

Abstract Image

Abstract Image

健康大鼠自动经颅光生物调节:对工作记忆和脑标记物的影响
光生物调节(PBM)利用红光和近红外光通过激活细胞色素c氧化酶(CCO)来刺激生物过程,促进ATP合成和神经保护。本研究评估了PBM对健康成年雄性大鼠空间工作记忆(WM)的影响和细胞机制,重点关注CCO活性、c-Fos和突触发生相关蛋白。PBM (810 nm, 40 Hz, 20 J cm−2)连续应用5天(PBM- c)或5天(PBM- a)。与对照组相比,PBM改善了两组的空间WM。前额叶和脾后皮层以及海马体的CCO活动减少,表明在认知任务中更有效地利用能量。PBM增加前额叶和顶叶皮层的c-Fos表达,反映了神经元活动的增强。在两种方案中,前额皮质突触素- 1水平升高,而PBM-C增加了海马体中的PSD-95。GFAP在皮质区域的表达均降低,而PBM-C在前额皮质的表达增加。这些发现表明,PBM- c增强了前额叶和海马突触,可能是观察到的WM改善的基础。本研究强调了PBM在调节CCO活性和突触可塑性方面的潜力,为确定WM保存和治疗的有效时间表和靶点提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Advanced Therapeutics
Advanced Therapeutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.10
自引率
2.20%
发文量
130
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信