Chemical Profiling of Anti-Inflammatory Constituents for Hongteng Baijiang Decoction Using Comprehensive Two-Dimensional Countercurrent Chromatography × High Performance Liquid Chromatography Coupled With Quadrupole Time-Of-Flight Mass Spectrometry

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Journal of separation science Pub Date : 2025-08-13 DOI:10.1002/jssc.70245

Hangjuan Lin, Wanshu Li, Yuli Gao, Bo Zhao, Bingqian Qin, Qiwen Shi, Chu Chu, Shengqiang Tong

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Abstract

Comprehensive two-dimensional reverse phase countercurrent chromatography (CCC)×high performance liquid chromatography (HPLC) was investigated for chemical profiling and identification of bioactive components with anti-inflammatory activities for a traditional Chinese medicine formula, Hongteng Baijiang decoction (HBD), for the first time. Anti-inflammatory activity of different extractions from HBD were evaluated. Two-dimensional contour plots for CCC×HPLC were obtained after comprehensive separation was completed. The total peak capacity of the two-dimensional system was evaluated, yielding approximately 782 peaks through comprehensive two-dimensional separation, which demonstrated a high degree of orthogonality and large separation space occupancy of 90.16%. Totally 207 chemical components were identified in ethyl acetate extract from HBD using liquid chromatography-quadrupole time-of-flight mass spectrometry based on the two-dimensional separation, with additional 70 compounds identified compared to that in one-dimensional HPLC separation. It was found that terpenoids were major bioactive components contributing to anti-inflammatory bioactivity in HBD. These findings highlight the potential of comprehensive two-dimensional chromatography (CCC×HPLC) as a powerful and promising method for separating and chemical profiling for complex traditional Chinese herbal formula.

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综合二维逆流色谱-高效液相色谱-四极杆飞行时间质谱分析红藤白姜汤抗炎成分

首次采用综合二维反相逆流色谱法（CCC）×high高效液相色谱法（HPLC）对中药复方红藤白姜汤（HBD）进行了化学分析和抗炎活性成分鉴定。对不同提取物的抗炎活性进行了评价。综合分离完成后得到CCC×HPLC的二维等高线图。评价了二维体系的总峰容，通过二维综合分离得到约782个峰，其正交度高，分离空间占用率达90.16%。采用液相色谱-四极杆飞行时间质谱法，在二维分离的基础上，共鉴定出207种化学成分，比一维高效液相色谱法多鉴定出70种化学成分。发现萜类化合物是HBD抗炎生物活性的主要生物活性成分。这些发现突出了综合二维色谱（CCC×HPLC）作为复杂中药配方分离和化学分析的一种强大而有前途的方法的潜力。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.