TRIB3 suppresses milk fatty acids metabolism by inhibiting p-AKT/PPARG signaling in goat mammary epithelial cells

Animal Research and One Health Pub Date : 2024-12-04 DOI:10.1002/aro2.98

Xinglong Gong, Tan Pan, Tiantian Xiong, Yifan Zhu, Juan J. Loor, Chengming Han, Yifan Li, Huan Lei, Jun Luo, Cong Li

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Abstract

Tribbles pseudokinase 3 (TRIB3) interacts with a variety of proteins and plays a key role in the regulation of glucose metabolism and glycolysis in nonruminants, but whether it has a specific role in goat mammary lipid metabolism has still been kept unknown. In this study, we observed that TRIB3 is highly expressed in the mammary tissues of lactating dairy goats. Overexpressing TRIB3 in goat mammary epithelial cells (GMECs) suppressed the mRNA expression of GPAM, DGAT1, and PLIN1, which are associated with the formation of triacylglycerol and lipid droplets (p < 0.05). The fatty acid-sensitive transcription regulator PPARG was also downregulated. Interfering TRIB3 had the opposite effect and decreased Akt phosphorylation. The TRIB3 gene influenced fatty acid composition in GMECs, and its overexpression reduced the total concentration of intracellular triacylglycerol (p < 0.01), this response was verified using BODIPY staining. Overall, these data indicated that TRIB3 suppresses milk fatty acids metabolism through inhibiting p-AKT/PPARG signaling in GMECs.

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TRIB3通过抑制山羊乳腺上皮细胞p-AKT/PPARG信号通路抑制乳脂肪酸代谢

Tribbles pseudokinase 3 （TRIB3）与多种蛋白质相互作用，在非反刍动物的糖代谢和糖酵解调节中起关键作用，但其是否在山羊乳腺脂质代谢中起特定作用尚不清楚。在本研究中，我们观察到TRIB3在哺乳期奶山羊的乳腺组织中高表达。山羊乳腺上皮细胞（gmac）过表达TRIB3可抑制与甘油三酯和脂滴形成相关的GPAM、DGAT1和PLIN1 mRNA的表达(p <；0.05)。脂肪酸敏感转录调控因子PPARG也下调。干扰TRIB3具有相反的效果，并降低Akt磷酸化。TRIB3基因影响gmec中脂肪酸组成，其过表达降低了细胞内甘油三酯的总浓度(p <；0.01)，用BODIPY染色证实了这种反应。综上所述，这些数据表明TRIB3通过抑制gmes中p-AKT/PPARG信号传导抑制乳脂肪酸代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Animal Research and One Health

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