The structure identification and antimicrobial prospective of copper nanoparticles derived via phyto-mediated synthesis using Eupatorium adenophorum

Abstract

The eco-friendly and sustainable character of the green synthesis of nanoparticles using plant-based materials has attracted significant attention. This investigation investigates the biosynthesis of copper nanoparticles (CuNPs) through the use of methanol extracts from Eupatorium adenophorum, an invasive plant that is abundant in bioactive phytochemicals. The plant extract's reduction of copper ions was visually detected by a distinct color change and subsequently verified through ultraviolet–visible (UV–Vis) spectroscopy. Functional groups that are responsible for the stabilization and capping of CuNPs were identified through Fourier transform infrared spectroscopy. Compared to the plant extract alone, the synthesized nanoparticles exhibited significantly larger inhibition zones against Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, and Micrococcus luteus, indicating potent antibacterial activity. These results underline the potential of E. adenophorum as a sustainable resource for nanoparticle synthesis, providing a dual benefit of repurposing an invasive species and contributing to green nanotechnology. This research points out the achievable applications of plant-mediated CuNPs in biomedical and environmental innovations.