Khaya grandifololia exerts multitarget neuroprotective potential against neurodegenerative disorders: In vitro and in silico studies

IF 2.9 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2025-08-12 DOI:10.1016/j.ibneur.2025.08.007

Fils Armand Ella, Bruno Dupon Akamba Ambamba, Frederic Nico Njayou, Paul Fewou Moundipa

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Abstract

Background

Treatment for complex multifactorial neurological disorders may benefit more from multifunctional chemicals. Dysregulation of monoaminergic pathways and neuroinflammation are typical confluence points in a range of neuropsychiatric and neurodegenerative illnesses. Polypharmacological medications that affect neuroinflammatory and monoaminergic pathways were investigated as potential targets for these diseases. The aim of this study was to investigate the in vitro and in silico multi-target neuroprotective activity of Khaya grandifololia.

Methods

Decoction and hydro-ethanolic extracts were prepared and screened for their ability to inhibit enzymes related to inflammation (15-lipoxygenase, LOX), neurodegeneration (monoamine oxidase, MAO), and protein glycation and fibrillation using enzymological fluorimetric assays and docking simulations.

Results

All extracts were able to strongly inhibit the activity of MAO as well as the glycation and fibrillation activities. Also, these extracts moderately inhibited 15-LOX activity.

Conclusion

These results extend the knowledge on the potential use of Khaya grandifololia to combat multifactorial disorders, giving new approaches into therapeutic avenues for neurodegenerative disease such as Alzheimer’s disease.

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山楂对神经退行性疾病具有多靶点神经保护潜力：体外和计算机研究

复杂的多因素神经系统疾病的治疗可能更受益于多功能化学物质。单胺能通路失调和神经炎症是一系列神经精神和神经退行性疾病的典型交汇点。影响神经炎症和单胺能通路的多种药物被研究作为这些疾病的潜在靶点。本研究旨在探讨桔梗的体外和体内多靶点神经保护活性。方法采用酶学荧光法和对接模拟，制备煎剂和水乙醇提取物，筛选其抑制炎症相关酶（15-脂氧合酶，LOX）、神经变性相关酶（单胺氧化酶，MAO）、蛋白质糖基化和纤维性颤动相关酶的能力。结果各提取物均能明显抑制MAO活性、糖基化活性和纤颤活性。此外，这些提取物适度抑制15-LOX活性。结论这些结果拓展了对大叶茶在治疗多因子疾病中的潜在应用的认识，为阿尔茨海默病等神经退行性疾病的治疗提供了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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