Investigating DEGs in developing insecticide resistance: Evidence of RpL40 and RpL27A inducing ubiquitin–PINK1-Parkin-dependent mitophagy pathway

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-08-10 DOI:10.1016/j.genrep.2025.102317

Srishti Sharma, Sujata Mohanty

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引用次数: 0

Abstract

Ethion (ET), an organophosphate (OP) insecticide acts by inhibiting acetylcholinesterase (AChE) leading to neurotoxicity and oxidative stress. However, its excessive and inappropriate use in agriculture and healthcare has resulted resistance in target-pest species and become harmful for non-target organisms. In this study, we investigated the development of ethion insecticide resistance in Drosophila melanogaster through biological and transcriptomic analysis and explored the molecular mechanisms that drive this adaptation process. Flies were exposed to two sublethal concentrations 1.25 ppm and 1.88 ppm of ethion for ten generations and F₁ and F₁₀ flies were analysed for the generational and transgenerational effect of ethion using biological parameters. Transcriptomic analysis was performed with the same generation flies (F₁ and F₁₀) to understand the differentially expressed genes (DEGs) and their involvement in various processes such as cell morphogenesis, transcription regulation, locomotor activities, metamorphosis, sensory and growth and visual system development, metabolism, catalytic and protein kinase activity etc. The comparison was made among two combinations of groups i.e., ethion F₁ treated (ETF₁) vs ethion-treated resistant (ETR) and control vs ETR. In control vs ETR group, heat shock ubiquitous DEGs were found to be upregulated which help organisms to cope up with insecticidal stress by refolding damaged proteins. The PPI network analysis revealed the key genes common to both the comparison groups and their involvement in various pathways. The up-regulated ubiquitin genes RpS27A and RPL40 evidenced the activation of ubiquitin-PINK1-Parkin-dependent mitophagy pathway in response to ethion-induced cellular stress.

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研究deg在产生杀虫剂抗性中的作用：RpL40和RpL27A诱导泛素- pink1 - parkin依赖性线粒体自噬途径的证据

乙硫磷（ET）是一种有机磷（OP）杀虫剂，通过抑制乙酰胆碱酯酶（AChE）导致神经毒性和氧化应激。然而，其在农业和卫生保健中的过度和不适当使用已导致目标害虫产生抗药性，并对非目标生物有害。在本研究中，我们通过生物学和转录组学分析研究了黑腹果蝇（Drosophila melanogaster）对乙硫磷杀虫剂抗性的发展，并探讨了驱动这一适应过程的分子机制。将蝇暴露于1.25 ppm和1.88 ppm两种亚致死浓度的硫醚中10代，并利用生物学参数分析F1和F10蝇的代际和跨代效应。对同代蝇（F1和F10）进行转录组学分析，了解差异表达基因（deg）及其在细胞形态发生、转录调控、运动活动、变态、感觉、生长和视觉系统发育、代谢、催化和蛋白激酶活性等过程中的作用。比较两组的组合，即F1处理组（ETF1）与耐药组（ETR）和对照组（ETR）。在对照组与ETR组中，热休克普遍存在的deg被发现上调，这有助于生物体通过重新折叠受损蛋白质来应对杀虫压力。PPI网络分析揭示了两个对照组共同的关键基因及其参与各种途径。泛素基因RpS27A和RPL40的上调证明了泛素- pink1 - parkin依赖的线粒体自噬途径在应激反应中被激活。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.