Influence of herbal extract blend supplementation on growth performance, liver and intestinal health, intestinal transcriptome, and resistance to Aeromonas veronii in Micropterus salmoides with optimized feeding intervals

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-08-08 DOI:10.1016/j.anifeedsci.2025.116467

Zi ying Zeng , Dong dong Yu , Yi Ting Shen, Xing han He, Shun Yang, Hui Fei

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引用次数: 0

Abstract

Our prior study has demonstrated that daily dietary supplementation with 1.0 g·kg⁻¹ herbal extracts (HE, comprising Scutellaria baicalensis, Taraxacum mongolicum, Biancaea sappan and Pulsatilla chinensis extract = 3.0: 1.8: 3.0: 1.7 (w/w/w/w)) can induce structural damage to the intestine, consequently impairing the growth performance of Micropterus salmoides. In the current study, we firstly investigated the effect of dietary HE (1.0 g·kg⁻¹) on resistance against Aeromonas veronii in M. salmoides with different post-feeding intervals, and found the highest survival rate was noted in the HSR group, where fish fed HE every three days, lasting for 28 days (phase I). Subsequently, the growth-related indexes, liver and intestinal health, intestinal transcriptome of fish in the HSR group was assayed (phase II). The results revealed that the feeding pattern in the HSR group had no significant impact on hepatic and liver morphology. However, it notably enhanced the growth rate, reduced malondialdehyde content, and decreased the activity of aspartate aminotransferase and alanine aminotransferase. Additionally, 275 upregulated and 183 downregulated genes were observed between the HSR and control groups by transcriptome analysis. The KEGG analysis illuminated that differentially expressed genes were predominantly annotated in endocytosis, lysosome, and MAPK signaling pathway. Among them, the growth-related genes, including fibroblast growth factor receptor 4-like, insulin-like growth factor I, alpha-galactosidase A, and beta-galactosidase- 1-like protein 2 were considerably upregulated, while the immune related genes, such as interleukin-1 beta, dual-specificity phosphatase 1, and dual-specificity phosphatase 2 was significantly downregulated. These results collectively illustrated the feeding pattern in the HSR group might be a potential approach to combat against A. veronii, as well as to mitigate the negative effect induced by intake of high level of HE for M. salmoides.

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优化饲喂间隔条件下，添加混合草药提取物对沙尾小虾生长性能、肝脏和肠道健康、肠道转录组以及对维罗氏气单胞菌抗性的影响

本研究表明，每天在饲料中添加1.0 g·kg−1的草药提取物（HE，由黄芩、蒲鲁蒿、白头草和白头草提取物= 3.0:1.8:3.0:1.7 （w/w/w/w）组成）可引起肠道结构损伤，从而影响小蝶的生长性能。在本研究中，我们首先研究了饲料中添加HE（1.0 g·kg−1）对不同投喂间隔salmoides对维氏气单胞菌抗性的影响，发现HSR组鱼的存活率最高，每3天投喂一次HE，持续28天（第一阶段）。随后，检测HSR组鱼的生长相关指标、肝脏和肠道健康状况、肠道转录组（II期）。结果显示，高铁组摄食方式对肝脏及肝脏形态无显著影响。但显著提高了生长速度，降低了丙二醛含量，降低了天冬氨酸转氨酶和丙氨酸转氨酶活性。此外，通过转录组分析，在HSR组和对照组之间观察到275个上调基因和183个下调基因。KEGG分析显示，差异表达基因主要在胞吞作用、溶酶体和MAPK信号通路中有注释。其中，成纤维细胞生长因子受体4样、胰岛素样生长因子I、α -半乳糖苷酶A、β -半乳糖苷酶-1样蛋白2等生长相关基因显著上调，而白细胞介素-1 β、双特异性磷酸酶1、双特异性磷酸酶2等免疫相关基因显著下调。这些结果共同表明，HSR组的摄食模式可能是对抗veronii的一种潜在方法，也可能减轻高水平HE摄入对salmoides的负面影响。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.