Antiviral RNA silencing in mammals: the importance of selecting the appropriate experimental model.

IF 13.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleic Acids Research Pub Date : 2025-08-11 DOI:10.1093/nar/gkaf743

Xing Liu,Yang Li

引用次数: 0

Abstract

The role of RNA interference (RNAi) in mammalian antiviral immunity remains debated, partly due to variations in experimental models. Here, we critically examine key factors influencing the detection and interpretation of antiviral RNAi, including virus selection, infection efficiency, and small RNA profiling. We highlight the limitations of certain viral models and the need for rigorous methodologies to distinguish canonical virus-derived small interfering RNAs from degradation products. Additionally, we discuss the challenges of using transgenic mouse models to study Dicer-mediated antiviral responses. Standardizing experimental approaches will be essential for clarifying the role of RNAi in mammalian antiviral defense.

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哺乳动物抗病毒RNA沉默：选择合适实验模型的重要性。

RNA干扰（RNAi）在哺乳动物抗病毒免疫中的作用仍然存在争议，部分原因是实验模型的差异。在这里，我们严格检查影响抗病毒RNAi检测和解释的关键因素，包括病毒选择，感染效率和小RNA分析。我们强调了某些病毒模型的局限性，以及需要严格的方法来区分典型病毒衍生的小干扰rna和降解产物。此外，我们讨论了使用转基因小鼠模型研究dicer介导的抗病毒反应的挑战。标准化实验方法对于阐明RNAi在哺乳动物抗病毒防御中的作用至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nucleic Acids Research 生物-生化与分子生物学

CiteScore

27.10

自引率

4.70%

发文量

1057

审稿时长

2 months

期刊介绍： Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.