Recreating fibroblast diversity in vitro by activating transcription factors

IF 29 1区生物学 Q1 GENETICS & HEREDITY

Nature genetics Pub Date : 2025-08-12 DOI:10.1038/s41588-025-02283-2

引用次数: 0

Abstract

We combined CRISPR activation of all 1,836 known human transcription factors with high-throughput Perturb-seq to recreate the diverse transcriptional states occupied by fibroblasts in vivo. Our study revealed regulators of key states and showed that inducing normal states can, in some cases, suppress those linked to disease.

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通过激活转录因子在体外重建成纤维细胞多样性

我们将所有1836个已知的人类转录因子的CRISPR激活与高通量Perturb-seq相结合，重现了体内成纤维细胞所占据的各种转录状态。我们的研究揭示了关键状态的调节因子，并表明在某些情况下，诱导正常状态可以抑制与疾病相关的状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature genetics 生物-遗传学

CiteScore

43.00

自引率

2.60%

发文量

241

审稿时长

3 months

期刊介绍： Nature Genetics publishes the very highest quality research in genetics. It encompasses genetic and functional genomic studies on human and plant traits and on other model organisms. Current emphasis is on the genetic basis for common and complex diseases and on the functional mechanism, architecture and evolution of gene networks, studied by experimental perturbation. Integrative genetic topics comprise, but are not limited to: -Genes in the pathology of human disease -Molecular analysis of simple and complex genetic traits -Cancer genetics -Agricultural genomics -Developmental genetics -Regulatory variation in gene expression -Strategies and technologies for extracting function from genomic data -Pharmacological genomics -Genome evolution