Acute toxic effects of an imidacloprid-based commercial insecticide in Caenorhabditis elegans

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-08-09 DOI:10.1016/j.etap.2025.104783

Julia Machado Menezes , Belisa Avila Rodrigues , Dora de Athayde Saul , Ariane da Silva Goulart , Matteus Teixeira Guerra , Daiana Silva Ávila , Karine Rigon Zimmer , Eliane Dallegrave

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引用次数: 0

Abstract

Imidacloprid (IMI), a neonicotinoid insecticide, targets postsynaptic nicotinic acetylcholine receptors, resulting in neurotoxicity in insects. Although selective for insects, IMI may have adverse effects on non-target species. This study investigated the acute toxicity of the commercial IMI formulation, Much 600 FS®, in Caenorhabditis elegans. L1-stage nematodes were acutely exposed to IMI at 2.4, 4.8, 7.2, 9.6, and 14.4 g/L concentrations. Developmental inhibition was observed at all concentrations, while significant mortality and reduced brood size occurred at 14.4 g/L. Behavioral assays revealed concentration-dependent decreases in pharyngeal pumping and locomotion. Cholinergic neuronal damage was evident at 7.2 g/L and above, indicating neurotoxicity. Our findings demonstrate that Much 600 FS® exerts toxic effects on C. elegans across developmental, behavioral, reproductive, and neuronal parameters, highlighting its potential risk to non-target organisms, even at sublethal levels.

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吡虫啉类商业杀虫剂对秀丽隐杆线虫的急性毒性作用。

吡虫啉（Imidacloprid， IMI）是一种新烟碱类杀虫剂，作用于突触后烟碱乙酰胆碱受体，对昆虫具有神经毒性。虽然IMI对昆虫具有选择性，但对非目标物种可能有不利影响。本研究考察了商业IMI制剂Much 600 FS®对秀丽隐杆线虫的急性毒性。l1期线虫急性暴露于2.4、4.8、7.2、9.6和14.4g/L浓度的IMI。在14.4g/L浓度下，雏鸟的发育均受到抑制，死亡率显著升高，雏鸟数量减少。行为分析显示浓度依赖性咽泵和运动减少。7.2g/L及以上胆碱能神经元损伤明显，提示神经毒性。我们的研究结果表明，Much 600 FS®对秀丽隐杆线虫的发育、行为、生殖和神经元参数都有毒性作用，突出了其对非目标生物的潜在风险，即使是在亚致死水平。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.