Multiple biochemical responses, osmoregulation, fatty acid composition, and vitamin E level in crayfish as biomarkers of exposure to thiacloprid and trifloxystrobin pesticides

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-08-09 DOI:10.1016/j.etap.2025.104788

Miraç Uçkun , Aysel Alkan Uçkun , Özden Barım Öz , Ertan Yoloğlu

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引用次数: 0

Abstract

Neonicotinoids and strobilurins are commonly used pesticides in agriculture, posing significant risks to aquatic animals. In this study, the toxic effects of thiacloprid and trifloxystrobin on Astacus leptodactylus were evaluated using various biomarkers, including acetylcholinesterase, carboxylesterase, glutathione S-transferase, glutathione reductase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, total antioxidant content, total oxidant scavenging capacity, adenosine 5’-TriPhospahatases, fatty acid composition, and vitamin E level. Both pesticides significantly affected the biomarkers compared to the control, activating and inhibiting them. Exposure to the pesticides led to an increase in monounsaturated and saturated fatty acids and a significant decrease in polyunsaturated fatty acids and vitamin E. The study’s findings suggest that thiacloprid and trifloxystrobin have harmful effects on A. leptodactylus through oxidative stress, detoxification, cholinergic, osmoregulation, and food quality systems. It is possible to conclude that these pesticides pose a major risk to public health as well as the aquatic ecology.

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小龙虾暴露于噻虫啉和三氯虫酯农药后的多种生化反应、渗透调节、脂肪酸组成和维生素E水平

新烟碱类和蝶柳素是农业中常用的农药，对水生动物构成重大风险。本研究以乙酰胆碱酯酶、羧酸酯酶、谷胱甘肽s -转移酶、谷胱甘肽还原酶、丙氨酸转氨酶、天冬氨酸转氨酶、乳酸脱氢酶、总抗氧化剂含量、总氧化剂清除能力、腺苷5′-三磷酸酶、脂肪酸组成和维生素E水平为指标，评价了噻虫啉和三氯虫酯对瘦肉Astacus leptodactylus的毒性。与对照相比，两种农药均显著影响生物标志物，激活或抑制它们。接触杀虫剂会导致单不饱和脂肪酸和饱和脂肪酸的增加，而多不饱和脂肪酸和维生素e的显著减少。研究结果表明，噻虫啉和三氯虫酯通过氧化应激、解毒、胆碱能、渗透调节和食品质量系统对细尾绦虫产生有害影响。可以得出结论，这些农药对公众健康和水生生态构成重大风险。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.