Silencing MMP2-AS1 inhibits the proliferation and migration of endothelial cells via YB-1.

IF 2.9 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Science Progress Pub Date : 2025-07-01 Epub Date: 2025-08-12 DOI:10.1177/00368504251366369

Zhetao Li, Kuo Wang, Ziliang Li, Yifan Chi

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Abstract

Objective: The processes of proliferation and migration in endothelial cells are fundamental to the development of atherosclerosis. Recent studies underscore the critical involvement of long noncoding RNAs (lncRNAs) in atherosclerosis by influencing the proliferation and migration of human umbilical vein endothelial cells (HUVECs). Nonetheless, the precise molecular mechanism of lncRNA matrix metalloproteinase 2 antisense RNA 1 (MMP2-AS1) remains inadequately characterized. Methods: To investigate this, we established a HUVEC model subjected to transforming growth factor-β1, oxidized low-density lipoprotein, and hypoxia, which resulted in the upregulation of lncRNA MMP2-AS1 expression. Further experimentation demonstrated that knockdown of MMP2-AS1 effectively mitigates the YB-1-mediated enhancement of HUVEC proliferation and migration. Results: Therefore, the primary aim of this research was to explore the role of the MMP2-AS1/YB-1 signaling pathway in atherosclerosis. The findings indicate that MMP2-AS1 regulates HUVEC proliferation and migration through YB-1 targeting. Conclusions: These results suggest that the MMP2-AS1/YB-1 axis holds potential as a therapeutic target for atherosclerosis treatment.

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沉默MMP2-AS1可通过YB-1抑制内皮细胞的增殖和迁移。

目的：血管内皮细胞的增殖和迁移过程是动脉粥样硬化发生发展的基础。最近的研究强调了长链非编码rna （lncRNAs）通过影响人脐静脉内皮细胞（HUVECs）的增殖和迁移而参与动脉粥样硬化的关键作用。尽管如此，lncRNA基质金属蛋白酶2反义RNA 1 （MMP2-AS1）的精确分子机制仍未得到充分的表征。方法：为此，我们建立了转化生长因子-β1、氧化低密度脂蛋白和缺氧导致lncRNA MMP2-AS1表达上调的HUVEC模型。进一步的实验表明，敲低MMP2-AS1可有效减轻yb -1介导的HUVEC增殖和迁移的增强。因此，本研究的主要目的是探讨MMP2-AS1/YB-1信号通路在动脉粥样硬化中的作用。结果表明，MMP2-AS1通过靶向YB-1调控HUVEC的增殖和迁移。结论：这些结果表明MMP2-AS1/YB-1轴具有作为动脉粥样硬化治疗靶点的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Progress Multidisciplinary-Multidisciplinary

CiteScore

3.80

自引率

0.00%

发文量

119

期刊介绍： Science Progress has for over 100 years been a highly regarded review publication in science, technology and medicine. Its objective is to excite the readers'' interest in areas with which they may not be fully familiar but which could facilitate their interest, or even activity, in a cognate field.