Safety and efficacy of a novel glycoengineered recombinant vaccine candidate against Haemonchus contortus in sheep.

IF 6.5 1区医学 Q1 IMMUNOLOGY

NPJ Vaccines Pub Date : 2025-08-11 DOI:10.1038/s41541-025-01249-z

Floriana Sajovitz-Grohmann, Isabella Adduci, Dirk Werling, Sandra Wiedermann, Licha N Wortha, Bojan Prole, Julia Zlöbl, Jolina Elster, Alexander Tichy, Anja Joachim, Thomas Wittek, Barbara Hinney, Shi Yan, Katharina Lichtmannsperger

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Abstract

Haemonchus contortus is a highly pathogenic nematode in small ruminants, causing major production losses and animal welfare concerns. With increasing anthelmintic resistance, vaccination offers a more sustainable control strategy. This study evaluated a novel glycoengineered vaccine produced in Hi5 insect cells in a randomized, controlled trial including Jura × Lacaune sheep. Animals received either Barbervax^® (BVAX), the glycoengineered vaccine (GEA) composed of five antigens (H11, H11-1, H11-2, H11-4 and GA1), its non-glycoengineered counterpart (NEA), or served as controls. Over 16 weeks, clinical, immunological, and parasitological data were collected. GEA reduced fecal egg counts by 81.09% and worm burden by 25.36%, showing a lower degree of anemia compared to NEA and control. All vaccinated groups exhibited elevated IgG/IgE responses. The results underscore the importance of glycoengineering in achieving protective immunity against H. contortus, supporting the development of highly effective recombinant vaccines against this and other parasitic worms in the future.

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一种新的抗绵羊弯曲血蜱糖工程重组候选疫苗的安全性和有效性。

弯曲血蜱是小反刍动物中一种高致病性线虫，造成重大生产损失和动物福利问题。随着驱虫抗药性的增加，疫苗接种提供了一种更可持续的控制策略。本研究在一项包括Jura × Lacaune羊的随机对照试验中评估了一种用Hi5昆虫细胞生产的新型糖工程疫苗。动物接种了Barbervax®（BVAX），由五种抗原（H11, H11-1, H11-2， H11-4和GA1）组成的糖工程疫苗（GEA），其非糖工程疫苗（NEA）或作为对照。在16周的时间里，收集临床、免疫学和寄生虫学数据。与NEA和对照组相比，GEA使粪卵数减少81.09%，虫量减少25.36%，贫血程度较低。所有接种组均表现出升高的IgG/IgE反应。这一结果强调了糖工程在获得对扭曲弧菌的保护性免疫中的重要性，为未来开发针对扭曲弧菌和其他寄生虫的高效重组疫苗提供了支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NPJ Vaccines Immunology and Microbiology-Immunology

CiteScore

11.90

自引率

4.30%

发文量

146

审稿时长

11 weeks

期刊介绍： Online-only and open access, npj Vaccines is dedicated to highlighting the most important scientific advances in vaccine research and development.