MAPIT-seq reveals both RBP targets and transcriptome-wide gene expression profiles

IF 32.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2025-08-11 DOI:10.1038/s41592-025-02775-3

引用次数: 0

Abstract

We present MAPIT-seq, a method that uses antibody-directed RNA editing to concurrently profile in situ RNA-binding protein (RBP)–RNA interactions and transcriptome-wide gene expression in limited input material, including single cells and frozen tissues. This dual-omic strategy streamlines mechanistic analyses of post-transcriptional regulation in dynamic biological processes and clinically relevant samples.

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MAPIT-seq揭示了RBP靶点和转录组范围内的基因表达谱。

我们提出MAPIT-seq，这是一种使用抗体定向RNA编辑的方法，可以在有限的输入材料（包括单细胞和冷冻组织）中同时分析原位RNA结合蛋白(RBP) -RNA相互作用和转录组全基因表达。这种双组学策略简化了动态生物过程和临床相关样本中转录后调控的机制分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.