Aspergillus awamori MH2 as a novel maltobionic acid producer: production optimization and application.

IF 4.9 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Cell Factories Pub Date : 2025-08-11 DOI:10.1186/s12934-025-02804-y

Soad M Yehia, Yousseria M Shetaia, Ghoson M Daba, Faten A Mostafa, Ali A Ali, Hassaan El-Menoufy

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引用次数: 0

Abstract

Background: Producing bio-based chemicals using a straightforward and ecologically responsible biotechnological method is intriguing. Maltobionic acid (MBA) is an aldobionic acid obtained from maltose oxidation and is an industrially applied compound. Having antioxidative and antimicrobial, non-toxic, highly water soluble, moisturizing, metal chelating, mildly sour and slightly sweet characteristics.

Results: This study succeeded in utilizing Aspergillus awamori as a novel MBA producer. MBA production was improved through two-step statistical factorial designs. Plackett-Burman Design (PBD) investigating the qualitative interaction between eleven factors (maltose, KNO₃, NaCl, KH₂PO₄, K₂HPO₄, MgSO₄, FeSO₄.7H₂O, initial pH, temperature, incubation time and rpm) on MBA production causing 1.37- fold increase. Central Composite design (CCD) analyzing the quantitative interaction between the most MBA production affecting factors (temperature, rpm, KH₂PO₄, and incubation period) gave a 1.64- improvement in MBA production compared with un-optimized medium. The addition of agro-industrial wastes (AIW) (corn cobs and artichoke leaves) to optimized medium g/l (maltose, 5; KNO₃, 1; KH₂PO₄, 1.0; MgSO₄, 0.5; FeSO₄.7(H₂O), 0.02; pH, 5.00 at 37.5 °C for 9.5 days at 125 rpm) caused 2.41- and 1.97- fold increase in MBA production, respectively in comparison with the initial production conditions. MBA produced by A. awamori MH2 exerted an anti-oxidant activity with a ratio of 86% using DPPH scavenging assay.

查看原文本刊更多论文

一种新型麦氏菌酸产菌awamori Aspergillus MH2：生产优化及应用。

背景：使用一种直接且对生态负责的生物技术方法生产生物基化学品是有趣的。麦芽糖仿生酸（MBA）是一种由麦芽糖氧化得到的醛仿生酸，是一种工业上应用的化合物。具有抗氧化抗菌、无毒、高水溶性、保湿、金属螯合、微酸微甜的特点。结果：本研究成功利用了青曲霉作为一种新型的MBA产菌。通过两步统计因子设计提高了MBA产量。Plackett-Burman设计（PBD）研究了11个因素（麦芽糖、KNO3、NaCl、KH2PO4、K2HPO4、MgSO4、FeSO4.7H2O、初始pH、温度、培养时间和转速）对MBA产量的定性相互作用，导致MBA产量增加1.37倍。中心复合设计（CCD）分析了大多数影响MBA产量的因素（温度、转速、KH2PO4和孵育时间）之间的定量交互作用，结果表明，与未优化的培养基相比，MBA产量提高了1.64-。在优化培养基g/l(麦芽糖，5；生产硝酸钾,1;KH2PO4 1.0;MgSO4 0.5;FeSO4.7 (H2O), 0.02;与初始生产条件相比，pH值为5.00（37.5°C, 125 rpm， 9.5天）的MBA产量分别增加了2.41倍和1.97倍。通过DPPH清除率测定，a . awamori MH2产生的MBA具有86%的抗氧化活性。

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来源期刊

Microbial Cell Factories 工程技术-生物工程与应用微生物

CiteScore

9.30

自引率

4.70%

发文量

235

审稿时长

2.3 months

期刊介绍： Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems