Yongyong Yan, Haiyan Wang, Huizhi Deng, Haokun He, Qing Ge, Jun Zha, Jun Chen, Qing Zhang, Haiyan Deng, Gang Wu, Richard T Jaspers, Janak L Pathak
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引用次数: 0
Abstract
Backgrounds: Periodontitis-induced alveolar bone loss is a primary cause of tooth loss. Porphyromonas gingivalis (P. gingivalis) is the primary pathogenic bacterium of periodontitis. Outer membrane vesicles (OMVs) derived from P. gingivalis (P.g-OMVs) contain various bioactive molecules, and several studies have suggested that P.g-OMVs may participate in alveolar bone loss caused by periodontitis.
Materials and methods: P.g-OMVs were isolated and characterized. The effect of P.g-OMVs on BMSCs proliferation and osteogenic differentiation was analyzed. High-throughput sequencing, RT-qPCR, and Western blot analysis were performed in BMSCs to unravel the underlying molecular mechanism.
Results: P.g-OMVs promoted proliferation but inhibited osteogenic differentiation of BMSCs. High-throughput sequencing results showed that serum amyloid A (SAA), especially SAA3, was robustly upregulated in P.g-OMVs-treated BMSCs. Upregulated SAA3 promoted TLR4, MyD88, and NF-κB p65 and inhibited osteogenic differentiation of P.g-OMVs-treated BMSCs. The knockdown of SAA3 in BMSCs downregulated P.g-OMVs-induced TLR4, MyD88, and NF-κB p65 and rescued P.g-OMVs-inhibited osteogenic differentiation.
Conclusions: Our results indicate that P.g-OMVs inhibit osteogenic differentiation of BMSCs via the SAA3-mediated TLR4/MyD88/NF-κB axis, providing novel targets for the treatment of periodontitis-induced alveolar bone loss.
期刊介绍:
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries