Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic Cells.

IF 3.6 3区 医学 Q2 IMMUNOLOGY
Journal of Immunology Research Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI:10.1155/jimr/2208155
Noémi Anna Nagy, Sanne G Celant, Toni M M van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B H Geijtenbeek, Sander W Tas, Esther C de Jong
{"title":"Intradermally Administered Retinoic Acid or Vitamin D3-Loaded Liposomes Induce Tolerogenic Skin Dendritic Cells.","authors":"Noémi Anna Nagy, Sanne G Celant, Toni M M van Capel, Rinske Sparrius, Fernando Lozano Vigario, Ronald van Ree, Bram Slütter, Teunis B H Geijtenbeek, Sander W Tas, Esther C de Jong","doi":"10.1155/jimr/2208155","DOIUrl":null,"url":null,"abstract":"<p><p>In vivo targeting of dendritic cells (DCs) with nanocarriers containing tolerogenic adjuvants is an attractive strategy to dampen inflammation. Here, we used ex vivo skin vaccination to examine the effect of intradermal injection of liposomes loaded with the tolerogenic adjuvants all-trans retinoic acid (RA) and vitamin D3 (VD3). We investigated the effect of intradermal liposome injection on skin DCs and the skin DC-induced T cell response. Our study shows that intradermal injection of RA or VD3-loaded anionic phospholipid 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) liposomes selectively induces CD14+ dermal DC (DDC) migration while reducing migration of CD1a dim DDCs. Migrated CD14+ DDCs displayed a partially immature phenotype. RA or VD3 liposome-treated CD1a dim DDCs exhibited reduced expression of maturation markers and induced expression of coinhibitory immunoglobulin-like transcript 3 (ILT3). VD3 liposome-treated CD14+ DDCs, as well as, CD1a dim DDCs, exhibited reduced expression of maturation markers, induction of coinhibitory molecules ILT3, and programmed death-ligand 1 (PD-L1). Migrated DCs from RA or VD3 liposome-injected skin differentiated naïve CD4+ T cells into FoxP3+ CD127 low and ICOS+ Tregs, expressing functional regulatory markers. Thus, our findings provide further substantiation for in vivo DC-modulating vaccines with tolerogenic liposomes as a putative clinical therapy for autoimmune diseases and allergies.</p>","PeriodicalId":15952,"journal":{"name":"Journal of Immunology Research","volume":"2025 ","pages":"2208155"},"PeriodicalIF":3.6000,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339165/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Immunology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/jimr/2208155","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In vivo targeting of dendritic cells (DCs) with nanocarriers containing tolerogenic adjuvants is an attractive strategy to dampen inflammation. Here, we used ex vivo skin vaccination to examine the effect of intradermal injection of liposomes loaded with the tolerogenic adjuvants all-trans retinoic acid (RA) and vitamin D3 (VD3). We investigated the effect of intradermal liposome injection on skin DCs and the skin DC-induced T cell response. Our study shows that intradermal injection of RA or VD3-loaded anionic phospholipid 1,2-distearoyl-sn-glycero-3-phosphoglycerol (DSPG) liposomes selectively induces CD14+ dermal DC (DDC) migration while reducing migration of CD1a dim DDCs. Migrated CD14+ DDCs displayed a partially immature phenotype. RA or VD3 liposome-treated CD1a dim DDCs exhibited reduced expression of maturation markers and induced expression of coinhibitory immunoglobulin-like transcript 3 (ILT3). VD3 liposome-treated CD14+ DDCs, as well as, CD1a dim DDCs, exhibited reduced expression of maturation markers, induction of coinhibitory molecules ILT3, and programmed death-ligand 1 (PD-L1). Migrated DCs from RA or VD3 liposome-injected skin differentiated naïve CD4+ T cells into FoxP3+ CD127 low and ICOS+ Tregs, expressing functional regulatory markers. Thus, our findings provide further substantiation for in vivo DC-modulating vaccines with tolerogenic liposomes as a putative clinical therapy for autoimmune diseases and allergies.

皮内给予维甲酸或维生素d3负载脂质体诱导耐受性皮肤树突状细胞。
在体内靶向树突状细胞(DCs)的纳米载体含有耐受性佐剂是一个有吸引力的策略,以减轻炎症。在这里,我们使用离体皮肤疫苗接种来研究皮内注射装载有耐受性佐剂全反式维甲酸(RA)和维生素D3 (VD3)的脂质体的效果。我们研究了皮内脂质体注射对皮肤dc的影响以及皮肤dc诱导的T细胞反应。我们的研究表明,皮内注射RA或负载vd3的阴离子磷脂1,2-二硬脂酰- san -甘油-3-磷酸甘油(DSPG)脂粒选择性地诱导CD14+真皮DC (DDC)迁移,同时减少CD1a暗淡DDC的迁移。迁移的CD14+ ddc表现出部分不成熟的表型。RA或VD3脂质体处理的CD1a暗淡ddc表现出成熟标志物的表达降低和诱导共抑制免疫球蛋白样转录物3 (ILT3)的表达。VD3脂质体处理的CD14+ ddc以及CD1a暗淡ddc表现出成熟标记物的表达降低,诱导共抑制分子ILT3和程序性死亡配体1 (PD-L1)。从RA或VD3脂质体注射皮肤中迁移的dc将naïve CD4+ T细胞分化为FoxP3+ CD127 low和ICOS+ treg,表达功能调节标志物。因此,我们的研究结果为体内dc调节疫苗与耐受性脂质体作为自身免疫性疾病和过敏的假定临床治疗提供了进一步的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.90
自引率
2.40%
发文量
423
审稿时长
15 weeks
期刊介绍: Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信