Evidence of ineffectiveness of insulin-like growth factor 1 (IGF-1) on in vitro production of bovine embryos: systematic review and meta-analysis.

IF 2.5 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Theriogenology Pub Date : 2025-12-01 Epub Date: 2025-08-07 DOI:10.1016/j.theriogenology.2025.117626
Giovana de Santi Phelippe Nunes, Alan Brunholi Giroto, Sarah Gomes Nunes, Edson Assunção Mareco, Beatriz Díaz-Fabregat, Anthony César de Souza Castilho
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引用次数: 0

Abstract

Unsatisfactory results of in vitro maturation (IVM) impair the development, survival and implantation of the embryo. Insulin-like growth factor 1 (IGF-1) is widely used in IVM, but its efficacy is controversial and consistent evidence is lacking. To investigate the efficacy of IGF-1 in IVM, we conducted a systematic review and meta-analysis on bovine oocytes competence and subsequent embryonic development. We structured the research question according to the PICO model: "Does IGF-1 enhance in vitro embryo production in cattle during maturation?" We consulted scientific databases such as PubMed/MEDLINE, Embase and Scopus. The assessment of the methodological quality of the selected articles, the risk of bias, was performed with SYRCLE® by two reviewers. Data analysis was performed with R software, the "META" package for meta-analysis, using the metabin function to assess data proportions and estimate random effects, and the forest function for graphical representation. Of the 43 articles evaluated, eight were included in the statistical analysis. The results of the meta-analysis showed no significant effect of IGF-1 on cleavage rate (OR = 1.28 [0.96; 1.71]; p > 0.05) and on blastocyst yield (OR = 0.88 [0.64; 1.22]; p > 0.05). In conclusion, although IGF-1 is a key protein for cell differentiation, its addition during IVM does not promote blastocyst development. Therefore, we propose novel strategies to increase the bioavailability of IGF-1 during IVM in cattle to potentially improve embryonic outcomes.

胰岛素样生长因子1 (IGF-1)对牛胚胎体外生产无效的证据:系统回顾和荟萃分析。
体外成熟(IVM)结果的不理想影响胚胎的发育、存活和着床。胰岛素样生长因子1 (IGF-1)广泛应用于IVM,但其疗效存在争议,缺乏一致的证据。为了研究IGF-1在IVM中的作用,我们对牛卵母细胞的能力和随后的胚胎发育进行了系统回顾和荟萃分析。我们根据PICO模型构建了研究问题:“IGF-1是否能提高成熟牛的体外胚胎产量?”我们参考了PubMed/MEDLINE、Embase和Scopus等科学数据库。所选文章的方法学质量评估和偏倚风险由两名审稿人使用sycle®进行。数据分析使用R软件进行META分析,使用META函数评估数据比例和估计随机效应,使用forest函数进行图形化表示。在评估的43篇文章中,有8篇被纳入统计分析。meta分析结果显示,IGF-1对卵裂率无显著影响(OR = 1.28 [0.96;1.71);p < 0.05)和囊胚产量(OR = 0.88 [0.64;1.22);p > 0.05)。综上所述,尽管IGF-1是细胞分化的关键蛋白,但在IVM期间添加IGF-1并不会促进囊胚发育。因此,我们提出了新的策略来提高牛IVM期间IGF-1的生物利用度,以潜在地改善胚胎结局。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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