TOR signalling mediates the collective movement of border cells in Drosophila oogenesis.

IF 3.6 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2025-09-01 Epub Date: 2025-09-04 DOI:10.1242/dev.204612

Sudipta Halder, Adhisree Sharma, Sayan Acharjee, Neha Biju, Mincy Kunjumon, Rohan Jayant Khadilkar, Mohit Prasad

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引用次数: 0

Abstract

Collective cell migration is seen in various biological processes spanning embryonic development, organogenesis, wound healing and, unfortunately, cancer metastasis. Here, we have examined the role of the evolutionarily conserved Target of Rapamycin signalling (TOR) in mediating collective cell movement employing the model of migrating border cells (BCs) in Drosophila oogenesis. Although TOR signalling is classically linked to cell growth, cell proliferation and metabolism, here we demonstrate that TOR complex 1 (TORC1) regulates efficient group cell movement of BCs. Employing live cell imaging, genetics, and tissue immunohistochemistry, we demonstrate that TOR functions through the transcription factor REPTOR to modulate Death-associated inhibitor of apoptosis 1 (Diap1) in mediating efficient movement of BCs. Coincidentally, rapamycin-treated myeloblast Kasumi-1 cells exhibit lower levels of transcript for the Diap1 homologue baculoviral IAP repeat-containing 2 (BIRC2), similar to what is observed in flies.

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TOR信号介导果蝇卵发生中边界细胞的集体运动。

在胚胎发育、器官发生、伤口愈合和癌症转移等多种生物过程中都可以看到集体细胞迁移。在这里，我们研究了进化保守的雷帕霉素信号传导靶点（TOR）在果蝇卵发生迁移bc模型中介导集体细胞运动中的作用。虽然TOR信号通常与细胞生长、细胞增殖和代谢有关，但在这里，我们证明了TOR复合物1 （TORC1）调节bc有效的群体细胞运动。利用活细胞成像、遗传学和组织免疫组织化学，我们证明了TOR通过转录因子Reptor调节死亡相关的凋亡抑制剂1 （DIAP1）在介导bc有效移动中的功能。巧合的是，雷帕霉素处理的成髓细胞Kasumi-1细胞表现出较低水平的DIAP-1同源物，杆状病毒IAP重复序列2 （BIRC2）的转录，与在苍蝇中观察到的相似。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

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