Advantages of ultrasonic-assisted extraction over hot water extraction for polysaccharides from waste stems of Rubia cordifolia L.: A comprehensive comparison of efficiency, structure, and bioactivity.

IF 9.7 1区 化学 Q1 ACOUSTICS
Ultrasonics Sonochemistry Pub Date : 2025-09-01 Epub Date: 2025-08-06 DOI:10.1016/j.ultsonch.2025.107502
Guanjun Ye, Jiangfei Li, Jiayu Wang, Ting Ju, Zhilong Zhou, Jianlong Wang, Haitian Zhao, Yingyu Zhou, Weihong Lu
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引用次数: 0

Abstract

Rubia cordifolia L. (RCL) is a plant with both commercial and medicinal values, widely distributed worldwide. Its roots have been used to treat various diseases, including abnormal uterine bleeding, arthritis, and kidney stones. However, its stems are often overlooked and regarded as waste. This study employed response surface methodology to optimize the ultrasonic-assisted extraction (UAE) process for polysaccharides from RCL waste stems (RCLP), and compared energy consumption and carbon emissions with hot water extraction (HWE). The underlying mechanism of UAE for polysaccharide extraction was further explored. Additionally, the physicochemical properties and in vitro activities of RCLP extracted by UAE (UAE-P) and HWE (HWE-P) were evaluated and compared. The results showed that the optimal UAE conditions were as follows: temperature of 52.5 °C, extraction time of 43 min, power of 310 W, and liquid-to-solid ratio of 32 mL/g, yielding 5.97 ± 0.34 % of RCLP. Compared with HWE, UAE significantly improved extraction efficiency, with a 640.18 % increase in production rate and a 93.23 % reduction in specific energy. UAE promoted the exposure of cell wall functional groups (e.g., C=O, C-O-C) and disintegration of the crystalline structure, thereby enhancing extraction efficiency. UAE-P exhibited higher uronic acid content, smaller and more uniform particle size, and better stability than HWE-P. These structural characteristics were significantly correlated with superior in vitro antioxidant and hypoglycemic activities, as well as stronger bile salt binding capacity. In conclusion, this study highlights that UAE is a feasible method for efficiently extracting high-value polysaccharides from RCL waste stems.

超声辅助提取与热水提取芦花废茎多糖的优势:效率、结构和生物活性的综合比较。
堇叶Rubia cordifolia L. (RCL)是一种具有商业和药用价值的植物,广泛分布在世界各地。它的根被用来治疗各种疾病,包括子宫异常出血、关节炎和肾结石。然而,它的茎往往被忽视,被视为浪费。采用响应面法对超声辅助提取RCL废茎多糖工艺进行了优化,并与热水提取工艺进行了能耗和碳排放比较。进一步探讨了UAE提取多糖的作用机理。此外,对UAE (UAE- p)和HWE (HWE- p)提取的RCLP的理化性质和体外活性进行了评价和比较。结果表明,最佳工艺条件为:提取温度52.5℃,提取时间43 min,提取功率310 W,液料比32 mL/g,得率为5.97±0.34%。与HWE相比,UAE显著提高了萃取效率,产率提高了640.18%,比能降低了93.23%。UAE促进了细胞壁官能团(如C=O, C-O-C)的暴露和晶体结构的分解,从而提高了提取效率。与HWE-P相比,UAE-P具有更高的醛酸含量、更小更均匀的粒径和更好的稳定性。这些结构特征与较好的体外抗氧化和降糖活性以及较强的胆盐结合能力显著相关。综上所述,UAE法是从RCL废茎中高效提取高价值多糖是一种可行的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Ultrasonics Sonochemistry
Ultrasonics Sonochemistry 化学-化学综合
CiteScore
15.80
自引率
11.90%
发文量
361
审稿时长
59 days
期刊介绍: Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.
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