Effect of particulate matter (PM10) on human neutrophils exposed to rhinovirus

IF 2.9 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Air Quality Atmosphere and Health Pub Date : 2025-05-20 DOI:10.1007/s11869-025-01719-2

Maria Camila Loaiza-Ceballos, Diana Maryory Gómez-Gallego, Juan C. Hernandez

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引用次数: 0

Abstract

Air pollutants seriously affect global health. Among these, particulate matter (PM10) can enter the respiratory tract, attract immune cells and cause inflammatory and cytotoxic phenomena that alter lung function and increase susceptibility to respiratory virus infections. Therefore, the objective of this study was to evaluate the inflammatory response induced by PM10 in neutrophils exposed to rhinovirus by evaluating its cytotoxic potential via the MTT technique and quantifying the levels of the proinflammatory cytokines IL-1β and TNF-α and their mRNAs of the ICAM-1 molecule using ELISA and qPCR, respectively. Furthermore, we evaluated the effect of PM10 exposure on the RV load in A549 cells. Exposure to PM10 at concentrations less than 10 µg/mL did not cause significant cytotoxicity in neutrophils, but when neutrophils were exposed to PM10 and Rhinovirus, increases in the levels of the IL-1β and ICAM-1 mRNA and the number of copies of the Rhinovirus genome, especially in neutrophils and cells exposed to higher concentrations of PM10. In conclusion, exposure to PM10 and rhinovirus may promote the occurrence of complications during viral infection related to the development of proinflammatory states.

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颗粒物（PM10）对暴露于鼻病毒的人中性粒细胞的影响

空气污染物严重影响全球健康。其中，颗粒物（PM10）可以进入呼吸道，吸引免疫细胞并引起炎症和细胞毒性现象，从而改变肺功能并增加对呼吸道病毒感染的易感性。因此，本研究的目的是通过MTT技术评估PM10对暴露于鼻病毒的中性粒细胞的细胞毒性，并分别使用ELISA和qPCR定量检测促炎细胞因子IL-1β和TNF-α及其ICAM-1分子mrna的水平，以评估PM10对暴露于鼻病毒的中性粒细胞的炎症反应。此外，我们评估了PM10暴露对A549细胞RV负荷的影响。暴露于浓度低于10 μ g/mL的PM10对中性粒细胞没有显著的细胞毒性，但当中性粒细胞暴露于PM10和鼻病毒时，IL-1β和ICAM-1 mRNA水平和鼻病毒基因组拷贝数增加，特别是在暴露于高浓度PM10的中性粒细胞和细胞中。综上所述，暴露于PM10和鼻病毒可能促进病毒感染期间与促炎状态发展相关的并发症的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Air Quality Atmosphere and Health ENVIRONMENTAL SCIENCES-

CiteScore

8.80

自引率

2.00%

发文量

146

审稿时长

>12 weeks

期刊介绍： Air Quality, Atmosphere, and Health is a multidisciplinary journal which, by its very name, illustrates the broad range of work it publishes and which focuses on atmospheric consequences of human activities and their implications for human and ecological health. It offers research papers, critical literature reviews and commentaries, as well as special issues devoted to topical subjects or themes. International in scope, the journal presents papers that inform and stimulate a global readership, as the topic addressed are global in their import. Consequently, we do not encourage submission of papers involving local data that relate to local problems. Unless they demonstrate wide applicability, these are better submitted to national or regional journals. Air Quality, Atmosphere & Health addresses such topics as acid precipitation; airborne particulate matter; air quality monitoring and management; exposure assessment; risk assessment; indoor air quality; atmospheric chemistry; atmospheric modeling and prediction; air pollution climatology; climate change and air quality; air pollution measurement; atmospheric impact assessment; forest-fire emissions; atmospheric science; greenhouse gases; health and ecological effects; clean air technology; regional and global change and satellite measurements. This journal benefits a diverse audience of researchers, public health officials and policy makers addressing problems that call for solutions based in evidence from atmospheric and exposure assessment scientists, epidemiologists, and risk assessors. Publication in the journal affords the opportunity to reach beyond defined disciplinary niches to this broader readership.