Nicholas Woytowich, Catherine Roberts, Phillip Ankley, Markus Brinkmann, Erik T. Krogh and Kyle D. Duncan*,
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引用次数: 0
Abstract
N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone (6PPDQ) is an emerging tire-derived contaminant that poses a critical environmental risk to select salmonids. However, the mechanisms of 6PPDQ toxicity are currently unresolved. While species-specific metabolomic effects of 6PPDQ exposure have been explored, to our knowledge no studies have investigated the in situ spatial distribution of 6PPDQ and 6PPDQ biotransformation products in salmonids. Herein, we employ nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging (MSI) to visualize 6PPDQ metabolism in 6-week posthatch rainbow trout fry exposed to 0.61 μg/L of 6PPDQ for 96 h. These data provide the first spatial mapping of 6PPDQ metabolism in fishes. 6PPDQ was not observed in any fish tissues; however, primary 6PPDQ biotransformation products, hydroxylated 6PPDQ and dihydroxylated 6PPDQ, were found to localize in the liver and gut of exposed fry. Additionally, interrogation of nontargeted nano-DESI spatial metabolomics revealed an accumulation of free fatty acids in the central nervous system of exposed fry. Overall, this study uncovers the spatially resolved 6PPDQ metabolism in exposed fishes and explores the disparate localization of endogenous metabolites disrupted by 6PPDQ exposure.
期刊介绍:
Environmental Science & Technology Letters serves as an international forum for brief communications on experimental or theoretical results of exceptional timeliness in all aspects of environmental science, both pure and applied. Published as soon as accepted, these communications are summarized in monthly issues. Additionally, the journal features short reviews on emerging topics in environmental science and technology.