Botanical origin and antimicrobial activity of bee pollen: Natural inhibitor for foodborne pathogens.

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-11-01 Epub Date: 2025-08-07 DOI:10.1016/j.micpath.2025.107978

Esra Deniz Candan, Duygu Nur Çobanoğlu, İlginç Kizilpinar Temizer

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引用次数: 0

Abstract

Introduction: Pollen collected by Apis mellifera is a product known for its various biological and therapeutic properties. This study aimed to evaluate the botanical origin, total phenolic content (TPC), total flavonoid content (TFC) and antimicrobial activity of bee pollen collected from Türkiye.

Methods: The botanical origin of bee pollen samples was analyzed using melissopalinology, TPC, TFC and their antibiomicrobial activities were analyzed by the disc diffusion method and minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC).

Results: 25 plant families, 44 genera, 5 species and 4 types were determined by palynological analysis. Two samples were monofloral (P2: Helianthus sp.; P6: Papaver sp.) and the others were heterofloral. TPC and TFC values varied within the range of 4.42 ± 0.02-14.68 ± 0.01 mg GAE/g and 1.1 ± 0.0010-3.4 ± 0.01 mg QE/g respectively. The antimicrobial resistance of bee pollen was investigated against ten foodborne pathogens, including Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella enterica), Gram positive bacteria (Bacillus cereus, Enterococcus faecalis, Listeria ivanovii, Listeria monocytogenes, Staphylococcus aureus), and yeast (Candida albicans). Pollen samples exhibited different resistance to all food pathogens. The results demonstrated that most of the bee pollen samples inhibited growth (48.4-67.2 %) the food pathogens tested.

Conclusions: It was found that the high antimicrobial effect of bee pollen samples against Enterococcus faecalis, Klebsiella pneumoniae, Listeria ivanovii, Pseudomonas aeruginosa, and Staphylococcus aureus. The study suggests that bee pollen could be a viable alternative for inhibiting the growth of foodborne pathogens. The present investigation is of significant regarding the usability of bee pollen as a natural antimicrobial agent for food preservation.

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蜂花粉的植物来源和抗菌活性：食源性病原体的天然抑制剂。

蜜蜂花粉是一种以其多种生物学和治疗特性而闻名的产品。本研究旨在对采自红叶蜂花粉的植物来源、总酚含量（TPC）、总黄酮含量（TFC）及抑菌活性进行评价。方法：采用微生物学方法对蜂花粉样品进行植物来源分析，采用纸片扩散法和最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、最小杀真菌浓度（MFC）分析其抗菌活性。结果：通过孢粉学分析鉴定出25科44属5种4型。2个样本为单花植物(P2: Helianthus sp.；P6: Papaver sp.)，其余为异花植物。TPC和TFC分别在4.42±0.02 ~ 14.68±0.01 mg GAE/g和1.1±0.0010 ~ 3.4±0.01 mg QE/g范围内变化。研究了蜂花粉对10种食源性病原菌的耐药性，包括革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、肠沙门氏菌）、革兰氏阳性菌（蜡样芽孢杆菌、粪肠球菌、伊万诺氏李斯特菌、单核增生李斯特菌、金黄色葡萄球菌）和酵母菌（白色念珠菌）。花粉样品对各种食物病原菌的抗性表现出不同程度的差异。结果表明，大多数蜂花粉样品对食品致病菌的生长有抑制作用（48.4% ~ 67.2%）。结论：蜂花粉样品对粪肠球菌、肺炎克雷伯菌、伊万诺氏李斯特菌、铜绿假单胞菌和金黄色葡萄球菌均有较高的抑菌效果。该研究表明，蜂花粉可能是抑制食源性病原体生长的可行替代品。本研究对蜂花粉作为天然抗菌剂用于食品保鲜具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)