Design of supports based on S-layer proteins-poly(acrylic) particles with varying surface properties

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-08-06 DOI:10.1016/j.colsurfb.2025.115014

Sofía Huggias , María A. Serradell , Mónica L. Casella , Patricia A. Bolla , Pablo J. Peruzzo

{"title":"Design of supports based on S-layer proteins-poly(acrylic) particles with varying surface properties","authors":"Sofía Huggias , María A. Serradell , Mónica L. Casella , Patricia A. Bolla , Pablo J. Peruzzo","doi":"10.1016/j.colsurfb.2025.115014","DOIUrl":null,"url":null,"abstract":"<div><div>This work proposes the study of the preparation of S-layer proteins-polymer particles supports by adsorption of the proteins on the polymer particles in aqueous dispersion. S-layer proteins were isolated from two <em>Lentilactobacillus kefiri</em> strains exhibiting different aggregative properties. Polyacrylic particles with similar sizes (around 70 nm) but different surface functionality (alkyl, hydroxylic, carboxylic), were obtained by emulsion polymerization and characterized. Combining them, adsorption isotherms were built. Adsorption isotherms follow the Langmuir-Freundlich model and show that the maximum amount of adsorbed protein was dependent on the protein/polymer combination. Both proteins showed a preference for particles containing carboxylic groups at the surface. Although the protein isolated from the non-aggregating strain presented the highest affinity for this surface, the protein obtained from the aggregating one led to the maximum amount of sorbed protein. The studies performed before and after sorption showed that the S-layer protein isolated from the aggregating strain presented a higher capacity to modify its secondary structure.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115014"},"PeriodicalIF":5.6000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloids and Surfaces B: Biointerfaces","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0927776525005211","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

This work proposes the study of the preparation of S-layer proteins-polymer particles supports by adsorption of the proteins on the polymer particles in aqueous dispersion. S-layer proteins were isolated from two Lentilactobacillus kefiri strains exhibiting different aggregative properties. Polyacrylic particles with similar sizes (around 70 nm) but different surface functionality (alkyl, hydroxylic, carboxylic), were obtained by emulsion polymerization and characterized. Combining them, adsorption isotherms were built. Adsorption isotherms follow the Langmuir-Freundlich model and show that the maximum amount of adsorbed protein was dependent on the protein/polymer combination. Both proteins showed a preference for particles containing carboxylic groups at the surface. Although the protein isolated from the non-aggregating strain presented the highest affinity for this surface, the protein obtained from the aggregating one led to the maximum amount of sorbed protein. The studies performed before and after sorption showed that the S-layer protein isolated from the aggregating strain presented a higher capacity to modify its secondary structure.

查看原文本刊更多论文

基于s层蛋白-具有不同表面特性的聚（丙烯酸）颗粒的支架设计

本工作提出了通过在水分散体中吸附蛋白质在聚合物颗粒上制备s层蛋白质-聚合物颗粒载体的研究。从两株具有不同聚集特性的克菲利慢乳杆菌中分离出s层蛋白。采用乳液聚合法制备了尺寸相近（约70 nm）但表面官能团（烷基、羟基、羧基）不同的聚丙烯酸颗粒，并对其进行了表征。结合它们，建立了吸附等温线。吸附等温线遵循Langmuir-Freundlich模型，并表明蛋白质的最大吸附量取决于蛋白质/聚合物的组合。两种蛋白质都偏爱表面含有羧基的颗粒。虽然从非聚集菌株中分离出的蛋白质对该表面的亲和力最高，但从聚集菌株中获得的蛋白质吸附量最大。吸附前后的研究表明，从聚集菌株中分离出的s层蛋白具有较高的二级结构修饰能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.