Molecular and physiological insights into nicotine degradation by Pseudomonas alloputida Pa_12: Implications for environmental management and tobacco processing

IF 10 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Cleaner Production Pub Date : 2025-08-11 DOI:10.1016/j.jclepro.2025.146220

Ang Li , Jie Zhang , Ao Li , Junaid Zafar , Ziling Wan , Yanqing Li , Rui Pang , Fengliang Jin , Xiaoxia Xu

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Abstract

The degradation and transformation of nicotine-containing environmental contaminants into harmless substances have become essential processes. In this study, we identified and characterized Pseudomonas alloputida strain Pa_12 (GenBank: CP158025), a bacterium with significant potential for the bioremediation of nicotine-contaminated environments. Comprehensive morphological, physiological and genomic analyses, including 16S rRNA sequencing, phylogenetic analysis, complete genome sequencing, and average nucleotide identity (ANI) comparison-confirmed the identity and metabolic capabilities of Pa_12. Under optimized conditions (pH 7, 30 °C), Pa_12 exhibited efficient nicotine catabolism. Absolute RT-qPCR and fluorescence in situ hybridization (FISH) demonstrated that Pa_12 successfully colonized tobacco leaves, promoted plant growth, and significantly reduced nicotine content in the foliage. LC-MS/MS analysis identified a nicotine catabolic pathway in Pa_12, distinct from the canonical pyrrole route, culminating in 2,5-dihydroxypyridine, thereby highlighting the unique capabilities of this strain. These findings advance our understanding of microbial nicotine degradation and present a viable solution for tobacco waste management and the improvement of flue-cured tobacco quality. Further investigation and development of Pa_12 may enable transformative applications in environmental remediation and sustainable tobacco cultivation.

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异臭假单胞菌Pa_12对尼古丁降解的分子和生理研究：对环境管理和烟草加工的意义

含尼古丁的环境污染物的降解和转化为无害物质已成为必不可少的过程。在本研究中，我们鉴定并鉴定了异臭假单胞菌菌株Pa_12 (GenBank: CP158025)，这是一种在尼古丁污染环境中具有重要生物修复潜力的细菌。综合形态学、生理学和基因组学分析，包括16S rRNA测序、系统发育分析、全基因组测序和平均核苷酸同源性（ANI）比较，证实了Pa_12的身份和代谢能力。在优化条件下（pH 7, 30°C）， Pa_12表现出高效的尼古丁分解代谢。绝对RT-qPCR和荧光原位杂交（FISH）结果表明，Pa_12成功定殖烟草叶片，促进植株生长，并显著降低烟叶中尼古丁含量。LC-MS/MS分析发现，Pa_12的尼古丁分解代谢途径不同于典型的吡咯途径，最终产生2,5-二羟基吡啶，从而突出了该菌株的独特能力。这些发现促进了我们对微生物尼古丁降解的理解，并为烟草废物管理和改善烤烟质量提供了可行的解决方案。Pa_12的进一步研究和开发可能会在环境修复和烟草可持续种植方面带来变革性的应用。

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来源期刊

Journal of Cleaner Production 环境科学-工程：环境

CiteScore

20.40

自引率

9.00%

发文量

4720

审稿时长

111 days

期刊介绍： The Journal of Cleaner Production is an international, transdisciplinary journal that addresses and discusses theoretical and practical Cleaner Production, Environmental, and Sustainability issues. It aims to help societies become more sustainable by focusing on the concept of 'Cleaner Production', which aims at preventing waste production and increasing efficiencies in energy, water, resources, and human capital use. The journal serves as a platform for corporations, governments, education institutions, regions, and societies to engage in discussions and research related to Cleaner Production, environmental, and sustainability practices.