Mechanism of Bushen Shengjing Decoction on the Spermatogenic Function of in Rats With Oligoasthenospermia: Focus on SCF/c-Kit Signaling Pathway-Mediated Apoptosis

IF 3 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Reproduction and Development Pub Date : 2025-08-11 DOI:10.1002/mrd.70044

Bo Chen, Hongmei He, Xiaoyan Sun, Qingyang Li, Qiaoyu Yang, Li Zhao, Xueling Li, Guanghui Zhou

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引用次数: 0

Abstract

This study investigated the effect of Bushen Shengjing Decoction (BSSJD) to modulate SCF/c-kit signaling pathway-mediated apoptosis on spermatogenesis in rats with oligoasthenospermia (OAS). The glucoside of Tripterygium wilfordii Hook F was given by gavage administration for OAS modeling in rats. The modeled rats were administered by gavage with BSSJD at different doses. Blood was collected from the abdominal aorta. The effect of BSSJD on OAS rats was determined by measuring serum testosterone (T), estradiol (E2) and luteinizing hormone (LH), epididymal index and testicular index, and sperm concentration and viability. Pathological changes in testicular tissues were observed. Caspase-3, Bax and Bcl-2 mRNA levels, along with SCF and c-kit levels in the testis tissues of rats were measured. BSSJD improved sperm concentration and quality, increased T and E2 levels, and reduced LH levels in rats with OAS. BSSJD alleviated apoptosis by decreasing Bax and caspase-3 and increasing Bcl-2 in testicular tissues of rats with OAS. Isck03 reversed the improvement of spermatogenesis and apoptosis in OAS rats treated with BSSJD. BSSJD improves spermatogenic function in OAS rats, which may act by regulating SCF/c-kit signaling pathway-mediated apoptosis.

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补肾生精汤对少弱精子症大鼠生精功能的影响机制：以SCF/c-Kit信号通路介导的细胞凋亡为研究重点

本研究探讨补肾生精汤（BSSJD）调节SCF/c-kit信号通路介导的凋亡对少弱精子症（OAS）大鼠精子发生的影响。采用雷公藤甙灌胃法造模大鼠OAS。采用不同剂量BSSJD灌胃造模大鼠。从腹主动脉采血。通过测定血清睾酮(T)、雌二醇（E2）、黄体生成素（LH）、附睾指数、睾丸指数、精子浓度和活力，观察BSSJD对OAS大鼠的影响。观察睾丸组织病理改变。测定大鼠睾丸组织中Caspase-3、Bax和Bcl-2 mRNA水平以及SCF和c-kit水平。BSSJD改善了OAS大鼠的精子浓度和质量，增加了T和E2水平，降低了LH水平。BSSJD通过降低OAS大鼠睾丸组织Bax和caspase-3，升高Bcl-2来减轻OAS大鼠睾丸组织的凋亡。Isck03逆转了BSSJD对OAS大鼠精子发生和细胞凋亡的改善。BSSJD改善OAS大鼠的生精功能，可能通过调节SCF/c-kit信号通路介导的细胞凋亡起作用。

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来源期刊

Molecular Reproduction and Development 生物-发育生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Molecular Reproduction and Development takes an integrated, systems-biology approach to understand the dynamic continuum of cellular, reproductive, and developmental processes. This journal fosters dialogue among diverse disciplines through primary research communications and educational forums, with the philosophy that fundamental findings within the life sciences result from a convergence of disciplines. Increasingly, readers of the Journal need to be informed of diverse, yet integrated, topics impinging on their areas of interest. This requires an expansion in thinking towards non-traditional, interdisciplinary experimental design and data analysis.