Bimetallic Fe3Cu-MOFs as Mimetic Enzyme and Fluorescent Probes for the Dual-Mode Detection of Uric Acid

IF 3.4 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Crystal Growth & Design Pub Date : 2025-07-09 DOI:10.1021/acs.cgd.5c00546

Jiali Zhong, Rou Cheng, Ziyi Guo, Zhixiang Yue, Zhengyue Xiao, Chunyan Yan, Xiaomin Tang and Ping Qiu*,

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Abstract

In recent years, the global burden of gout has been increasing, and an excessively high level of blood uric acid is an important cause of gout attacks. The rapid detection of uric acid (UA) is of great significance for the auxiliary diagnosis of disease. We synthesized bifunctional Fe₃Cu-MOFs that combined excellent peroxidase activity and fluorescence probe. UA reacts with uricase to generate H₂O₂, which could be catalyzed by the Fe₃Cu-MOF to involve hydroxyl radicals (^•OH) and singlet oxygen (¹O₂). And these free radicals can oxidize o-phenylenediamine (OPD) to 2,3-diaminophenazine (DAP) and the solution changes from colorless to yellow. Meanwhile, there is fluorescence resonance energy transfer (FRET) between DAP and Fe₃Cu-MOF, resulting in the weakening of the blue fluorescence of Fe₃Cu-MOF. Based on these mechanisms, we constructed a dual-mode sensor for UA detection. The detection ranges of the colorimetric method and the ratiometric fluorescence method of this sensor are 1–250 and 0.5–250 μM, respectively, and the limits of detection are 0.43 and 0.30 μM, respectively. These results indicate that the sensor has excellent sensitivity for UA detection. In addition, we have also successfully applied this sensor to the detection of UA content in human serum samples and urine samples.

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双金属Fe3Cu-MOFs作为模拟酶和荧光探针用于尿酸双模检测

近年来，全球痛风负担不断加重，血尿酸水平过高是痛风发作的重要原因。尿酸（UA）的快速检测对疾病的辅助诊断具有重要意义。我们合成了双功能Fe3Cu-MOFs，它结合了优异的过氧化物酶活性和荧光探针。UA与尿酸酶反应生成H2O2，由Fe3Cu-MOF催化生成羟基自由基（•OH）和单线态氧（1O2）。这些自由基可以将邻苯二胺（OPD）氧化为2,3-二氨基苯那嗪（DAP），溶液由无色变为黄色。同时，DAP与Fe3Cu-MOF之间存在荧光共振能量转移（FRET），导致Fe3Cu-MOF的蓝色荧光减弱。基于这些机制，我们构建了用于UA检测的双模传感器。该传感器比色法和比例荧光法的检测范围分别为1 ~ 250 μM和0.5 ~ 250 μM，检出限分别为0.43 μM和0.30 μM。结果表明，该传感器具有良好的UA检测灵敏度。此外，我们还成功地将该传感器应用于人体血清样本和尿液样本中UA含量的检测。

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来源期刊

Crystal Growth & Design 化学-材料科学：综合

CiteScore

6.30

自引率

10.50%

发文量

650

审稿时长

1.9 months

期刊介绍： The aim of Crystal Growth & Design is to stimulate crossfertilization of knowledge among scientists and engineers working in the fields of crystal growth, crystal engineering, and the industrial application of crystalline materials. Crystal Growth & Design publishes theoretical and experimental studies of the physical, chemical, and biological phenomena and processes related to the design, growth, and application of crystalline materials. Synergistic approaches originating from different disciplines and technologies and integrating the fields of crystal growth, crystal engineering, intermolecular interactions, and industrial application are encouraged.