Sidneia Sanches de Menezes Costa, Beatriz Girardo, Thamy Caroline Silva, Karen Ziza, Glaciano Ribeiro, Dante M Langhi, Carla Luana Dinardo, José O Bordin
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引用次数: 0
Abstract
Background: ABO antibodies can activate complement and cause hemolysis, sometimes with ABO minor incompatible platelet or plasma transfusions. Donor low titres of anti-ABO are used to assess transfusion safety. However, the correlation between antibody titres (measured semi-quantitatively or quantitatively) and their ability to activate complement is unclear.
Objectives: This study aimed to correlate ABO antibody (IgM/IgG) titres and the presence of IgG1/IgG3 subclasses with complement-mediated haemolysis using the CHUHE-P assay (Complement Hemolysis Using Human Erythrocytes).
Methods: Single-center blood group O donor samples were tested with semi-quantitative methods and classified in group 1 as low ABO titres if titre <100 and into group 2 as high ABO titre if titre >100. Testing included IgM/IgG antibody titration, the CHUHE-P assay, haemolysin test, and determination of IgG1/IgG3 subclasses.
Results: Group 1 had 21 and Group 2 had 56 blood donors. In Group 2, CHUHE-P positivity was not associated with IgM or IgG anti-A/B titres or haemolysin test results. IgG1 and IgG3 subclasses of anti-A were significantly associated with positive CHUHE-P. Significant differences between groups included CHUHE-P positivity (p < 0.001), IgG titres (p < 0.01) for anti-A, and CHUHE-P positivity (p < 0.001), IgM titres (p = 0.04), and haemolysin test (p = 0.02) for anti-B. In Group 1, 33% of anti-A and 23.8% of anti-B had positive CHUHE-P.
Conclusions: This study aimed to compare the ability of anti-ABO antibodies to cause complement-mediated hemolysis measured by the CHUHE-P test and the results of semi-quantitative/quantitative titration assays, the presence of IgG1 and/or IgG3, and hemolysin test results, where we highlighted an inconsistent association between the methods; the positivity for CHUHE-P was associated with the subclasses of antibodies IgG1 and IgG3.
期刊介绍：
Transfusion Medicine publishes articles on transfusion medicine in its widest context, including blood transfusion practice (blood procurement, pharmaceutical, clinical, scientific, computing and documentary aspects), immunohaematology, immunogenetics, histocompatibility, medico-legal applications, and related molecular biology and biotechnology.
In addition to original articles, which may include brief communications and case reports, the journal contains a regular educational section (based on invited reviews and state-of-the-art reports), technical section (including quality assurance and current practice guidelines), leading articles, letters to the editor, occasional historical articles and signed book reviews. Some lectures from Society meetings that are likely to be of general interest to readers of the Journal may be published at the discretion of the Editor and subject to the availability of space in the Journal.
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