In Vivo Effects of Papaya Seed Essential Oil on Systemic Candidiasis Model Mice Induced by Fluconazole-Sensitive and -Resistant Isolates of Candida albicans.

IF 2.9 3区生物学 Q2 MYCOLOGY

Mycopathologia Pub Date : 2025-08-09 DOI:10.1007/s11046-025-00976-0

Yinzheng Ma, Mingqi Yu, Jianing Qian, Bingzhangke Bao, Xinyi Ma, Zijie Li, Zibei Wu, Yuzhong Jia, Xiaowen He

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引用次数: 0

Abstract

Systemic candidiasis caused by Candida albicans has become a serious issue due to high morbidity and mortality. It is very important to develop novel and effective antifungal agents due to the increasing resistance to commonly used drugs such as fluconazole (FLC). In our previous research, the essential oil of Carica papaya L. seed (PSEO) was extracted and in vitro studies demonstrated that PSEO exhibited strong antifungal activity against C. albicans. It is crucial to investigate the therapeutic effect of PSEO in vivo to develop it into a viable antifungal agent. This study aimed to investigate acute toxicity of PSEO and its in vivo effects on a systemic candidiasis model in mice induced with both FLC-sensitive and -resistant C. albicans. The results showed that PSEO showed no acute toxicity up to 100 mg/kg. PSEO and FLC can improve the water intake, food intake, and mental state of systemic candidiasis model mice. The body weight of the infected mice recovered, the survival rate and splenic index increased, the fungal burden decreased, and pathological damage was alleviated to varying degrees. Moreover, the higher the dose of PSEO, the more pronounced the effect. PSEO exhibited significantly better efficacy than FLC against FLC-resistant C. albicans-induced systemic candidiasis. These findings indicate that PSEO holds great promise as a natural, novel, and effective agent for clinical treatment of C. albicans infections, especially for drug-resistant C. albicans infections. This study provides a theoretical basis for the rational development and utilization of papaya seeds.

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木瓜籽精油对氟康唑敏感和耐药白色念珠菌诱导的全身念珠菌病模型小鼠的体内影响

白色念珠菌引起的全身性念珠菌病由于其高发病率和死亡率已经成为一个严重的问题。由于对氟康唑（FLC）等常用药物的耐药性日益增加，开发新型有效的抗真菌药物非常重要。我们在前期的研究中提取了番木瓜种子精油（Carica papaya L. seed精油，PSEO），体外实验表明，PSEO对白色念珠菌具有较强的抗真菌活性。为了使其成为一种有效的抗真菌药物，研究其在体内的治疗效果至关重要。本研究旨在探讨PSEO的急性毒性及其对flc敏感和耐药白色念珠菌诱导的全身念珠菌病模型的体内影响。结果表明，当浓度达到100 mg/kg时，PSEO无急性毒性。pso和FLC能改善全身性念珠菌病模型小鼠的饮水、摄食量和精神状态。感染小鼠体重恢复，存活率和脾指数升高，真菌负担减轻，病理损伤均有不同程度减轻。此外，pso剂量越高，效果越明显。pso对耐FLC白色念珠菌诱导的全身性念珠菌病的治疗效果明显优于FLC。这些发现表明，PSEO作为一种天然的、新型的、有效的白色念珠菌感染的临床治疗药物具有很大的前景，特别是对于耐药的白色念珠菌感染。本研究为木瓜种子的合理开发利用提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycopathologia 生物-真菌学

CiteScore

6.80

自引率

3.60%

发文量

审稿时长

3 months

期刊介绍： Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.