Race-related host and microbe transcriptomic signatures in triple-negative breast cancer.

IF 7.6 2区医学 Q1 ONCOLOGY

NPJ Breast Cancer Pub Date : 2025-08-08 DOI:10.1038/s41523-025-00806-y

Roshan Kumar, Susan Duyar-Ayerdi, Aishwarya Sundaresan, Vinodh Srinivasasainagendra, Chandra Sekhar Pedamallu, Michael Behring, Darshan Shimoga Chandrashekar, Isam-Eldin Eltoum, Sooryanarayana Varambally, Hemant K Tiwari, Sadeep Shrestha, Paul L Auer, Lubna N Chaudhary, John R Kirby, Clayton Yates, Upender Manne, Akinyemi I Ojesina

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Abstract

Triple-negative breast cancer (TNBC) shows racial disparities, with higher incidence in women of African ancestry (AA) compared to European ancestry (EA). Meta-transcriptomic analysis of TNBC tumor tissues from AA (n = 17) and EA (n = 19) subjects revealed distinct microbial landscapes. Hierarchical clustering based on microbial transcripts separated samples into two groups predominantly defined by racial ancestry. Bacterial genera including Hafnia and Cedecea were more abundant in AA tumors, while Erwinia was higher in EA tumors. Cellular composition analysis by xCell revealed differences in immune cell populations, with AA tumors having higher Th1 cell abundance and EA tumors containing higher macrophage M2 cell abundance. Nonetheless, AA women with high M2 abundance experienced poorer disease-free survival (DFS) than EA women. Integrative analyses revealed that high expression of human SPDYE2B gene was associated with Hafnia abundance and decreased DFS, highlighting complex host-microbe interactions in TNBC patients.

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三阴性乳腺癌中与种族相关的宿主和微生物转录组特征

三阴性乳腺癌（TNBC）表现出种族差异，与欧洲血统（EA）相比，非洲血统（AA）女性的发病率更高。来自AA （n = 17）和EA （n = 19）受试者的TNBC肿瘤组织的meta转录组学分析显示了不同的微生物景观。基于微生物转录本的分层聚类将样品分为两组，主要由种族血统定义。AA肿瘤中细菌属Hafnia和Cedecea较多，EA肿瘤中细菌属Erwinia较多。xCell细胞组成分析显示免疫细胞群存在差异，AA肿瘤具有较高的Th1细胞丰度，EA肿瘤具有较高的巨噬细胞M2细胞丰度。尽管如此，M2丰度高的AA女性比EA女性的无病生存期（DFS）更差。综合分析显示，人类spdy2b基因的高表达与Hafnia丰度和DFS降低有关，突出了TNBC患者中复杂的宿主-微生物相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NPJ Breast Cancer Medicine-Pharmacology (medical)

CiteScore

10.10

自引率

1.70%

发文量

122

审稿时长

9 weeks

期刊介绍： npj Breast Cancer publishes original research articles, reviews, brief correspondence, meeting reports, editorial summaries and hypothesis generating observations which could be unexplained or preliminary findings from experiments, novel ideas, or the framing of new questions that need to be solved. Featured topics of the journal include imaging, immunotherapy, molecular classification of disease, mechanism-based therapies largely targeting signal transduction pathways, carcinogenesis including hereditary susceptibility and molecular epidemiology, survivorship issues including long-term toxicities of treatment and secondary neoplasm occurrence, the biophysics of cancer, mechanisms of metastasis and their perturbation, and studies of the tumor microenvironment.