Peroxiredoxin 4 (Prx 4) plays important gene expression regulatory roles in antimicrobial innate immune by regulating hydrogen peroxide (H2O2) in the hepatopancreas of freshwater crayfish Procambarus clarkii

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-08-06 DOI:10.1016/j.dci.2025.105438

Jing Wen , Shuyao Liu , Bingbing Yang , Mingyue Tan , Xiuli Shen , Zhiqiang Du

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引用次数: 0

Abstract

This study focused on peroxiredoxin (Prx) as a core target to investigate its regulatory mechanism in the innate immunity of Procambarus clarkii (crayfish). We observed that a reduction in the expression of prx 4 via injection of double-stranded RNA (dsRNA) induced early upregulation of H₂O₂ levels in the hepatopancreas of crayfish. When the crayfish were subsequently infected with Vibrio harveyi, the H₂O₂ levels further increased, and the antioxidant enzyme system was activated. After injecting dsPc-Prx 4 and subsequently stimulating the crayfish with V. harveyi, we observed upregulated expression of genes related to the melanization pathway, reactive oxygen species (ROS) pathway, apoptosis pathway, and Toll pathway.

Furthermore, after the injection of dsPc-Prx 4 followed by V. harveyi and subsequent N-acetylcysteine (NAC) exposure, the expression of genes related to the melanization, apoptosis, and Toll pathways was downregulated; moreover, the melanization phenomenon was significantly weakened, and the survival rate of the crayfish decreased.

The abovementioned experimental results demonstrate that Pc-Prx 4 is an important regulatory enzyme in the antioxidant system of crayfish. It can influence the antibacterial innate immune response of P. clarkii by modulating H₂O₂ levels. This study provides a significant addition to the fundamental theory of antibacterial innate immunity in invertebrates and offers a theoretical basis for the prevention and control of bacterial diseases in P. clarkii.

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过氧化物氧还蛋白4 （Prx 4）通过调控克氏原螯虾肝胰腺过氧化氢（H2O2）在抗微生物先天免疫中发挥重要的基因表达调控作用。

本研究以过氧化物还氧蛋白（Prx）为核心靶点，探讨其在克氏原螯虾（Procambarus clarkii）先天免疫中的调控机制。我们观察到，通过注射双链RNA （dsRNA）降低prx4的表达可诱导小龙虾肝胰腺中H2O2水平的早期上调。当小龙虾随后感染哈维弧菌时，H2O2水平进一步升高，抗氧化酶系统被激活。在注射dsp - prx 4后，用V. harveyi刺激小龙虾，我们观察到黑色素化途径、活性氧（ROS）途径、凋亡途径和Toll途径相关基因的表达上调。此外，注射dsPc-Prx 4后，V. harveyi和随后的n -乙酰半胱氨酸（NAC）暴露后，黑色素化、凋亡和Toll通路相关基因的表达下调；黑化现象明显减弱，小龙虾存活率下降。上述实验结果表明，Pc-Prx 4是小龙虾抗氧化系统中重要的调节酶。它可以通过调节H2O2水平影响克氏假单胞菌的抗菌先天免疫反应。本研究为无脊椎动物抗菌先天免疫的基础理论提供了重要补充，为克氏杆菌细菌性疾病的预防和控制提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.