Effect of ART on Reverting the Drug-Resistance of SGC7901/mdr1 and Reducing the Anti-Apoptosis of Bcl-2.

IF 0.6 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2025-08-01 DOI:10.7754/Clin.Lab.2025.241236

Xianhui Wang, Yinxiu Chi, Zhiyong Wang

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引用次数: 0

Abstract

Background: Many studies have shown that artesunate (ART) has an anti-tumor effect, but its effect on gastric cancer is not clear. The purpose of the study was to observe the Adriamycin (ADM)-resistance changes of SGC 7901/mdr1 caused by ART, compare it with SGC7901, and explore the cell apoptosis and changes of Bcl-2's expression in the reversal process.

Methods: MTT was used to test ART's drug-resistance reversal effect. Fluorescence microscope and flow cytometry were applied to test the apoptosis of SGC7901/mdr1 cells. Western blotting was used to test the expressions of Bcl-2 and caspase-3.

Results: It was found that non-cytotoxic ART combined with ADM in different concentrations can produce intensified damage to SGC7901/mdr1. The resistance reversal ratio of ART to drug resistant cells was about 2.31 times, and the relative reversal rate was about 83%. After 48h combination of non-cytotoxic ART and non-cytotoxic ADM, cell apoptosis was observed in morphology. The results of FC showed that the apoptosis ratio of combined group reached 15%. The results of Western blot suggested that Bcl-2's expressions in the combined group were significantly enhanced in comparison to the single drug group, and activation fragments of caspase-3 were observed.

Conclusions: ART can reverse doxorubicin resistance in drug-resistant cells, and the mechanism of reversal is related to the increase of apoptosis.

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ART对逆转SGC7901/mdr1耐药及降低Bcl-2抗凋亡的影响

背景：许多研究表明青蒿琥酯（ART）具有抗肿瘤作用，但其对胃癌的作用尚不清楚。本研究旨在观察ART引起的SGC7901 /mdr1对阿霉素（ADM）耐药性的变化，并与SGC7901进行比较，探讨逆转过程中细胞凋亡及Bcl-2表达的变化。方法：采用MTT法检测ART的耐药逆转效果。采用荧光显微镜和流式细胞术检测SGC7901/mdr1细胞的凋亡情况。Western blotting检测Bcl-2和caspase-3的表达。结果：无细胞毒性ART联合不同浓度的ADM可对SGC7901/mdr1产生强化损伤。ART对耐药细胞的耐药逆转率约为2.31倍，相对逆转率约为83%。无细胞毒性ART与无细胞毒性ADM联合48h后，形态学上观察到细胞凋亡。FC结果显示，联合组细胞凋亡率达15%。Western blot结果显示，与单药组相比，联合用药组Bcl-2的表达明显增强，并观察到caspase-3的活化片段。结论：ART可逆转耐药细胞对阿霉素的耐药性，其逆转机制与细胞凋亡增加有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.