Association of Silent Variant p.17Pro = SNP in the LHX4 Gene with Litter Size in Awassi Sheep.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2025-08-09 DOI:10.1007/s10528-025-11223-y

Tahreer M Al-Thuwaini, Ahmed H Alkhammas, Fadhil A Rhadi, Alaa H Kadhim

{"title":"Association of Silent Variant p.17Pro = SNP in the LHX4 Gene with Litter Size in Awassi Sheep.","authors":"Tahreer M Al-Thuwaini, Ahmed H Alkhammas, Fadhil A Rhadi, Alaa H Kadhim","doi":"10.1007/s10528-025-11223-y","DOIUrl":null,"url":null,"abstract":"<p><p>LIM homeobox transcription factor 4 (LHX4) is one of the genes involved in sheep reproduction. Consequently, this study investigated whether the LHX4 gene affects the litter size of ewes. Genomic DNA extraction was performed on 123 ewes with singleton lambs and 109 ewes with twins. Using polymerase chain reaction (PCR), fragments of 207, 256, 257, 325, and 377 bp were amplified from exons 1, 2, 3, 5, and 6 of the LHX4 gene. Genetic analysis of the amplified locus of 207 bp revealed three genotypes. An analysis of the sequence revealed a novel mutation in exon 1: p.17Pro = SNP. Statistical analysis showed that the p.17Pro = SNP was associated with reproductive characteristics (P ≤ 0.01). Ewes carrying the GG genotype had significantly lower live body weight, litter sizes, twinning rates, lambing rates, and more lambing days than ewes with AG and AA genotypes. Lambs produced by GG genotype ewes were fewer than those produced by AA and AG genotype ewes. These results indicate that the p.17Pro = SNP negatively affects Awassi sheep's reproductive traits. Ewes carrying the GG genotype are less prolific and have lower litter sizes than those without the SNP.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10528-025-11223-y","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

LIM homeobox transcription factor 4 (LHX4) is one of the genes involved in sheep reproduction. Consequently, this study investigated whether the LHX4 gene affects the litter size of ewes. Genomic DNA extraction was performed on 123 ewes with singleton lambs and 109 ewes with twins. Using polymerase chain reaction (PCR), fragments of 207, 256, 257, 325, and 377 bp were amplified from exons 1, 2, 3, 5, and 6 of the LHX4 gene. Genetic analysis of the amplified locus of 207 bp revealed three genotypes. An analysis of the sequence revealed a novel mutation in exon 1: p.17Pro = SNP. Statistical analysis showed that the p.17Pro = SNP was associated with reproductive characteristics (P ≤ 0.01). Ewes carrying the GG genotype had significantly lower live body weight, litter sizes, twinning rates, lambing rates, and more lambing days than ewes with AG and AA genotypes. Lambs produced by GG genotype ewes were fewer than those produced by AA and AG genotype ewes. These results indicate that the p.17Pro = SNP negatively affects Awassi sheep's reproductive traits. Ewes carrying the GG genotype are less prolific and have lower litter sizes than those without the SNP.

查看原文本刊更多论文

阿瓦西羊LHX4基因沉默变异p.17Pro = SNP与产仔数的关系

LIM同源盒转录因子4 （LHX4）是绵羊生殖相关基因之一。因此，本研究探讨了LHX4基因是否会影响母羊的产仔数。对123只单胎母羊和109只双胞胎母羊进行了基因组DNA提取。采用聚合酶链反应（PCR）技术，从LHX4基因的第1、2、3、5和6外显子中扩增出207、256、257、325和377 bp的片段。对扩增位点207bp进行遗传分析，发现3种基因型。对该序列的分析显示，外显子1出现了一个新的突变：p.17Pro = SNP。统计分析显示，P . 17pro = SNP与繁殖性状相关（P≤0.01）。GG基因型母羊的活重、产仔数、双胞胎率、产羔率和产羔天数均显著低于AG和AA基因型母羊。GG基因型母羊的产仔量少于AA和AG基因型母羊。这些结果表明，p.17Pro = SNP对阿瓦西羊的生殖性状有负向影响。与没有SNP的母羊相比，携带GG基因型的母羊繁殖能力较差，产仔数也较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.