SERS-based miniaturized biosensors for alkaline phosphatase detection: Towards intelligent, real-time diagnostics in precision medicine.

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-11-15 Epub Date: 2025-08-05 DOI:10.1016/j.bios.2025.117793

Ajitesh Dhal, Ana Elena Aviña, Cheng-Jen Chang, Chang-I Chen, Tzu Sen Yang

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引用次数: 0

Abstract

Alkaline phosphatase (ALP) is a clinically important hydrolase enzyme and a valuable biomarker for hepatobiliary diseases, metabolic bone disorders, and certain malignancies. Raman-based miniaturized sensors, particularly those employing surface-enhanced Raman scattering (SERS), have enabled ultrasensitive and selective ALP detection at femtomolar to picomolar levels in complex biological samples. This narrative review critically examines recent advances in SERS-enabled ALP sensors, highlighting hotspot engineering, nanozyme-assisted signal amplification, and microfluidic integration to achieve high-throughput, low-volume assays. It also explores the incorporation of artificial intelligence algorithms for real-time spectral interpretation and discusses the potential for integrating these systems with fifth and sixth generation (5G/6G) wireless networks for rapid, cloud-based diagnostics. In addition, this review outlines current challenges, including substrate reproducibility and standardization issues, and proposes strategies to enhance clinical translation. Collectively, these developments are transforming ALP sensing by enabling decentralized, intelligent, and personalized diagnostic platforms, which hold promise for advancing precision healthcare and improving patient outcomes.

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用于碱性磷酸酶检测的基于sers的微型生物传感器：在精准医学中实现智能、实时诊断。

碱性磷酸酶（ALP）是临床上重要的水解酶，也是肝胆疾病、代谢性骨疾病和某些恶性肿瘤的有价值的生物标志物。基于拉曼的小型化传感器，特别是那些采用表面增强拉曼散射（SERS）的传感器，已经能够在复杂生物样品的飞摩尔到皮摩尔水平上进行超灵敏和选择性的ALP检测。这篇综述批判性地考察了SERS-enabled ALP传感器的最新进展，重点介绍了热点工程、纳米酶辅助信号放大和微流体集成，以实现高通量、小体积的检测。该报告还探讨了人工智能算法在实时光谱解释中的应用，并讨论了将这些系统与第五代和第六代（5G/6G）无线网络集成以实现快速云诊断的潜力。此外，本文概述了当前的挑战，包括底物可重复性和标准化问题，并提出了加强临床翻译的策略。总的来说，这些发展正在通过启用分散、智能和个性化的诊断平台来改变ALP传感，这些诊断平台有望推进精确医疗保健并改善患者的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.