Arsenite antiporters ACR3/3;1 are critical for Se-enhanced plant growth and As accumulation in As-hyperaccumulators Pteris multifida and Pteris cretica.

IF 11.3
Journal of hazardous materials Pub Date : 2025-09-15 Epub Date: 2025-08-06 DOI:10.1016/j.jhazmat.2025.139434
Wei Li, Si-Xue He, Yi-Wen Liu, Qian-Yu Zhou, Shu-Fen Xiao, Yanshan Chen, Yue Cao, Lena Q Ma
{"title":"Arsenite antiporters ACR3/3;1 are critical for Se-enhanced plant growth and As accumulation in As-hyperaccumulators Pteris multifida and Pteris cretica.","authors":"Wei Li, Si-Xue He, Yi-Wen Liu, Qian-Yu Zhou, Shu-Fen Xiao, Yanshan Chen, Yue Cao, Lena Q Ma","doi":"10.1016/j.jhazmat.2025.139434","DOIUrl":null,"url":null,"abstract":"<p><p>Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As<sub>50</sub>) plus 1.25 (Se<sub>1.25</sub>) or 2.5 μM (Se<sub>2.5</sub>) selenate under hydroponics. After 2 weeks of growth, the biomass, As, Se and malondialdehyde (MDA) contents, and important genes related to As metabolism in two plants were determined. Both plants were able to take up and translocate As to the fronds, with Se-enhanced plant growth being observed only under As exposure. For both plants, the As+Se treatments increased their biomass by 22-32 % compared to the As<sub>50</sub> treatment, which may be attributed to 42-45 % reduction in the MDA contents. However, the effects of Se on plant growth and As accumulation varied with Se doses and plant species. For P. multifida, 1.25 µM Se enhanced the As content by 52 % to 329 mg kg<sup>-1</sup> in the fronds, while 2.5 µM Se decreased. For P. cretica, Se promoted its frond As contents by 42-106 % to 155-225 mg kg<sup>-1</sup>, with 2.5 μM being more effective. Though showing little effect on phosphate transporter Pht1 or arsenate reductase HAC1, Se induced upregulation of arsenite antiporters ACR3/3;1 by 1.6-3.0 fold, which promoted As translocation from the roots to fronds and As sequestration into the fronds under As exposure. Se effectively enhances plant growth and As accumulation in P. multifida and P. cretica by upregulating the expression of arsenite antiporters ACR3. Based on literature and our data, ACR3/3;1 play critical roles in Se-enhanced As accumulation in As-hyperaccumulators, which may have application in phytoremediation of As-contaminated soils in temperate zones.</p>","PeriodicalId":94082,"journal":{"name":"Journal of hazardous materials","volume":"496 ","pages":"139434"},"PeriodicalIF":11.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of hazardous materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.jhazmat.2025.139434","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/6 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Selenium (Se) enhances plant growth and As accumulation in As-hyperaccumulator Pteris vittata, but its effects on As-hyperaccumulators Pteris multifida and Pteris cretica are unknown. Here, they were exposed to 50 μM arsenate (As50) plus 1.25 (Se1.25) or 2.5 μM (Se2.5) selenate under hydroponics. After 2 weeks of growth, the biomass, As, Se and malondialdehyde (MDA) contents, and important genes related to As metabolism in two plants were determined. Both plants were able to take up and translocate As to the fronds, with Se-enhanced plant growth being observed only under As exposure. For both plants, the As+Se treatments increased their biomass by 22-32 % compared to the As50 treatment, which may be attributed to 42-45 % reduction in the MDA contents. However, the effects of Se on plant growth and As accumulation varied with Se doses and plant species. For P. multifida, 1.25 µM Se enhanced the As content by 52 % to 329 mg kg-1 in the fronds, while 2.5 µM Se decreased. For P. cretica, Se promoted its frond As contents by 42-106 % to 155-225 mg kg-1, with 2.5 μM being more effective. Though showing little effect on phosphate transporter Pht1 or arsenate reductase HAC1, Se induced upregulation of arsenite antiporters ACR3/3;1 by 1.6-3.0 fold, which promoted As translocation from the roots to fronds and As sequestration into the fronds under As exposure. Se effectively enhances plant growth and As accumulation in P. multifida and P. cretica by upregulating the expression of arsenite antiporters ACR3. Based on literature and our data, ACR3/3;1 play critical roles in Se-enhanced As accumulation in As-hyperaccumulators, which may have application in phytoremediation of As-contaminated soils in temperate zones.

亚砷酸盐反转运蛋白ACR3/3;1对硒增强植物生长和砷超富集植物Pteris multifida和Pteris cretica的砷积累至关重要。
硒(Se)能促进超富集As植物Pteris vittata的生长和As积累,但对超富集As Pteris multifida和Pteris cretica的影响尚不清楚。在这里,他们在水培下暴露于50 μM砷酸盐(As50)加1.25 (Se1.25)或2.5 μM (Se2.5)硒酸盐。生长2周后,测定两株植株的生物量、砷、硒和丙二醛(MDA)含量以及与砷代谢相关的重要基因。这两种植物都能吸收砷并将其转运到叶片上,只有在砷暴露下才观察到硒促进植物生长。与As50处理相比,As+Se处理使两种植物的生物量增加了22-32 %,这可能是由于MDA含量降低了42-45 %。硒对植物生长和砷积累的影响随硒剂量和植物种类的不同而不同。1.25 µM硒可使多裂叶子中As含量提高52. %,达到329 mg kg-1,而2.5 µM硒可使多裂叶子中As含量降低。硒能使叶面As含量提高42 ~ 106 %,达到155 ~ 225 mg kg-1,其中以2.5 μM的效果更好。虽然硒对磷转运体Pht1和砷酸盐还原酶HAC1的影响不大,但硒能诱导亚砷酸盐反转运体ACR3/3;1上调1.6 ~ 3.0倍,促进砷暴露下砷从根向叶的转运和砷在叶中的固存。硒通过上调亚砷酸盐反转运蛋白ACR3的表达,有效地促进了多假假和白芷的生长和砷积累。根据文献和我们的数据,ACR3/3;1在硒增强As在As超积累体中的积累中起关键作用,可能在温带As污染土壤的植物修复中有应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信