Exploring the impact of IKZF1 and its targeting microRNAs in the pathogenesis of acute lymphoblastic leukemia.

IF 1.6 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Irish Journal of Medical Science Pub Date : 2025-08-08 DOI:10.1007/s11845-025-04065-5

Ilia Heidari, Ashraf Kariminik

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引用次数: 0

Abstract

Background: Acute lymphoblastic leukemia (ALL) is an aggressive hematologic malignancy driven by uncontrolled immature lymphoid cell proliferation. Its prognosis is significantly influenced by IKZF1 gene alterations which contribute to treatment resistance and relapse.

Aim: This study investigates the role of specific IKZF1-targeting microRNAs (e.g., hsa-miR-488, hsa-miR-4311) in ALL pathogenesis and their potential as therapeutic targets.

Methods: Peripheral blood samples were collected from 32 ALL patients and 20 healthy controls. Total RNA and miRNAs were extracted, and cDNA was synthesized. Quantitative real-time PCR was performed to evaluate the expression of IKZF1 and the candidate miRNAs. Statistical analyses included non-parametric tests to compare expression levels between groups.

Results: IKZF1 expression was significantly reduced in ALL patients compared to controls (median: 0.1160 vs. 0.8245; p = 0.002). Among the miRNAs analyzed, only hsa-miR-8085 showed a significant decrease in ALL patients (median: 0.0192 vs. 0.3411; p = 0.008). No significant differences were observed for hsa-miR-488, hsa-miR-4311, hsa-miR-6731-5p, or hsa-miR-6833-3p.

Conclusions: The significant downregulation of IKZF1 and hsa-miR-8085 in ALL patients suggests their involvement in the development of leukemia and highlights their potential as diagnostic markers or therapeutic targets.

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探讨IKZF1及其靶向microrna在急性淋巴细胞白血病发病机制中的作用。

背景：急性淋巴细胞白血病（Acute lymphoblastic leukemia， ALL）是一种不受控制的未成熟淋巴细胞增殖引起的侵袭性血液系统恶性肿瘤。其预后受到IKZF1基因改变的显著影响，从而导致治疗抵抗和复发。目的：本研究探讨特异性ikzf1靶向microrna（如hsa-miR-488、hsa-miR-4311）在ALL发病机制中的作用及其作为治疗靶点的潜力。方法：采集32例ALL患者外周血和20例健康对照。提取总RNA和mirna，合成cDNA。实时荧光定量PCR检测IKZF1及候选mirna的表达情况。统计分析包括非参数检验来比较各组之间的表达水平。结果：与对照组相比，ALL患者的IKZF1表达显著降低(中位数：0.1160 vs 0.8245；p = 0.002)。在所分析的mirna中，只有hsa-miR-8085在ALL患者中表现出显著降低(中位数：0.0192 vs. 0.3411；p = 0.008)。hsa-miR-488、hsa-miR-4311、hsa-miR-6731-5p或hsa-miR-6833-3p无显著差异。结论：ALL患者中IKZF1和hsa-miR-8085的显著下调表明它们参与了白血病的发展，并突出了它们作为诊断标志物或治疗靶点的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Irish Journal of Medical Science 医学-医学：内科

CiteScore

3.70

自引率

4.80%

发文量

357

审稿时长

4-8 weeks

期刊介绍： The Irish Journal of Medical Science is the official organ of the Royal Academy of Medicine in Ireland. Established in 1832, this quarterly journal is a contribution to medical science and an ideal forum for the younger medical/scientific professional to enter world literature and an ideal launching platform now, as in the past, for many a young research worker. The primary role of both the Academy and IJMS is that of providing a forum for the exchange of scientific information and to promote academic discussion, so essential to scientific progress.